Spring 2012:LFM Literature

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20.345: Bioinstrumentation Project Lab

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Light field imaging allows for the capture of the light field of a sample in a single photograph.[1] This has been recently commercialized in the Lytro Camera[2] which obtains a light field using a microlens and allows for processing of the image afterwards.
Light Field Microscope Diagram from the Stanford 2006 paper.[3]

They also documented their setup in more detail in a technical memo.[4]

Introduction to LFDisplay.[5]

Light field rendering.[6]

In 2009, the group published a better quality machine 4D Microscopy[7]


I would be thrilled to work on this project for a number of reasons:

  • It will force me to work on image processing, I am novice status :p
  • It would be an awesome project
  • It is feasible
    • Only one additional physical component in a traditional bright field microscope
    • Lots of code
  • It is relevant
    • This microscope can be used to compose a 3-dimensional image of the sample
    • Taking 3-dimensional images quickly is a current point of interest in microscopy today
  • It is novel
    • With respect to this class, this is a novel project
    • Otherwise, this is not a new technology [6] but has been implemented into prototype microscopes in the past 7 years


  1. A practical introduction to light field microscopy - Stanford 2010 (pdf)
  2. Light field camera
  3. Light field microscopy - Stanford 2006 (pdf)
  4. Optical recipes for light field microscopes - Stanford 2006 (pdf)
  5. LFDisplay: A real time system for light field microscopy
  6. 6.0 6.1 Light field rendering - Stanford 1996
  7. Recording and controlling the 4D light field in a microscope - Stanford 2009 (pdf)