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  1. 20.109(F17):Complete in silico cloning (Day1)
  2. 20.109(F17):Confirm gRNA sequence (Day5)
  3. 20.109(F17):Design gRNA for CRISPRi (Day2)
  4. 20.109(F17):Develop experiment to optimize loading variables (Day2)
  5. 20.109(F17):Evaluate cell loading results (Day3)
  6. 20.109(F17):Examine sub-nuclear foci abundance to measure DNA damage (Day6)
  7. 20.109(F17):Generate gRNA plasmid (Day3)
  8. 20.109(F17):Growth of phage materials (Day1)
  9. 20.109(F17):Homework
  10. 20.109(F17):Induce CRISPRi system (Day7)
  11. 20.109(F17):Journal club II (Day 6)
  12. 20.109(F17):Journal club I (Day4)
  13. 20.109(F17):Lab tour
  14. 20.109(F17):Module 1
  15. 20.109(F17):Module 2
  16. 20.109(F17):Module 3
  17. 20.109(F17):Prepare comet chips (Day1)
  18. 20.109(F17):Purify active materials (Day2)
  19. 20.109(F17):Research proposal presentation
  20. 20.109(F17):Test role of biochemical factors in genomic stability (Day4)
  21. 20.109(F17):Visualize active material using TEM (Day4)
  22. 20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)
  23. 20.109(F17): Announcements
  24. 20.109(F17): Assignments
  25. 20.109(F17): Biomaterials engineering mini-report
  26. 20.109(F17): Fall 2017 Schedule
  27. 20.109(F17): M1 Data Summary
  28. 20.109(F17): M1 Mini-presentation
  29. 20.109(F17): Notes for M1
  30. 20.109(F17): Notes for orientation
  31. 20.109(F17): Research article
  32. 20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)
  33. 20.109(F18):Assemble and test batteries (Day5)
  34. 20.109(F18):Class data
  35. 20.109(F18):Communication
  36. 20.109(F18):Complete genomic instability experiment and load cells for sub-nuclear foci assay (Day4)
  37. 20.109(F18):Complete in silico cloning (Day1)
  38. 20.109(F18):Complete sub-nuclear foci assay (Day6)
  39. 20.109(F18):Confirm gRNA sequence (Day5)
  40. 20.109(F18):Construct cathode with phage-based active material (Day3)
  41. 20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)
  42. 20.109(F18):Design gRNA for CRISPRi (Day2)
  43. 20.109(F18):Evaluate cell loading experiment and treat cells for genomic instability experiment (Day3)
  44. 20.109(F18):Generate gRNA plasmid (Day3)
  45. 20.109(F18):Growth of phage-based active (cathode) material (Day1)
  46. 20.109(F18):Homework
  47. 20.109(F18):Image sub-nuclear foci assay
  48. 20.109(F18):Image sub-nuclear foci assay (Day6)
  49. 20.109(F18):Induce CRISPRi system (Day7)
  50. 20.109(F18):Journal club presentation (Day4 and 6)
  51. 20.109(F18):Lab tour
  52. 20.109(F18):Measure fermentation products (Day8)
  53. 20.109(F18):Module 1
  54. 20.109(F18):Module 2
  55. 20.109(F18):Module 3
  56. 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7)
  57. 20.109(F18):Practice tissue culture and prepare microwell array (Day1)
  58. 20.109(F18):Prep notes for M3
  59. 20.109(F18):Prepare and treat cells for genomic instability experiment (Day3)
  60. 20.109(F18):Purify phage-based active (cathode) material (Day2)
  61. 20.109(F18):Research proposal presentation
  62. 20.109(F18):Visualize phage nanowires using TEM (Day4)
  63. 20.109(F18): Announcements
  64. 20.109(F18): Assignments
  65. 20.109(F18): Data Summary
  66. 20.109(F18): Fall 2018 schedule
  67. 20.109(F18): Mini-presentation
  68. 20.109(F18): Mini-report
  69. 20.109(F18): Prep notes for M1
  70. 20.109(F18): Prep notes for M2
  71. 20.109(F18): Research article
  72. 20.109(F19):Begin Western blot analysis (Day3)
  73. 20.109(F19):Class data
  74. 20.109(F19):Communication
  75. 20.109(F19):Complete CometChip experiment (Day5)
  76. 20.109(F19):Complete antibody staining for Western blot analysis (Day4)
  77. 20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)
  78. 20.109(F19):Complete in silico cloning (Day1)
  79. 20.109(F19):Complete in silico cloning of pdCas9 (Day1)
  80. 20.109(F19):Confirm gRNA sequence in pgRNA(Day5)
  81. 20.109(F19):Confirm gRNA sequence in pgRNA (Day5)
  82. 20.109(F19):Data analysis and assignment preparation (Day5)
  83. 20.109(F19):Design gRNA for CRISPRi (Day2)
  84. 20.109(F19):Design gRNA sequence for CRISPRi (Day2)
  85. 20.109(F19):Generate gRNA plasmid (Day3)
  86. 20.109(F19):Guidelines for working in the tissue culture facility
  87. 20.109(F19):Homework
  88. 20.109(F19):Image CometChip (Day6)
  89. 20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)
  90. 20.109(F19):Induce CRISPRi system (Day7)
  91. 20.109(F19):Journal club presentation (Day4 and 6)
  92. 20.109(F19):Lab tour
  93. 20.109(F19):Load cells into CometChip and apply treatments (Day4)
  94. 20.109(F19):Measure fermentation products (Day8)
  95. 20.109(F19):Module 1
  96. 20.109(F19):Module 2
  97. 20.109(F19):Module 3
  98. 20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)
  99. 20.109(F19):Practice statistical analysis methods and complete data analysis (Day7)
  100. 20.109(F19):Prepare for cellular thermal shift assay (Day1)

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