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- 20.109(F17):Complete in silico cloning (Day1)
- 20.109(F17):Confirm gRNA sequence (Day5)
- 20.109(F17):Design gRNA for CRISPRi (Day2)
- 20.109(F17):Develop experiment to optimize loading variables (Day2)
- 20.109(F17):Evaluate cell loading results (Day3)
- 20.109(F17):Examine sub-nuclear foci abundance to measure DNA damage (Day6)
- 20.109(F17):Generate gRNA plasmid (Day3)
- 20.109(F17):Growth of phage materials (Day1)
- 20.109(F17):Homework
- 20.109(F17):Induce CRISPRi system (Day7)
- 20.109(F17):Journal club II (Day 6)
- 20.109(F17):Journal club I (Day4)
- 20.109(F17):Lab tour
- 20.109(F17):Module 1
- 20.109(F17):Module 2
- 20.109(F17):Module 3
- 20.109(F17):Prepare comet chips (Day1)
- 20.109(F17):Purify active materials (Day2)
- 20.109(F17):Research proposal presentation
- 20.109(F17):Test role of biochemical factors in genomic stability (Day4)
- 20.109(F17):Visualize active material using TEM (Day4)
- 20.109(F17):Visualize and analyze data for sub-nuclear foci assay (Day7)
- 20.109(F17): Announcements
- 20.109(F17): Assignments
- 20.109(F17): Biomaterials engineering mini-report
- 20.109(F17): Fall 2017 Schedule
- 20.109(F17): M1 Data Summary
- 20.109(F17): M1 Mini-presentation
- 20.109(F17): Notes for M1
- 20.109(F17): Notes for orientation
- 20.109(F17): Research article
- 20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)
- 20.109(F18):Assemble and test batteries (Day5)
- 20.109(F18):Class data
- 20.109(F18):Communication
- 20.109(F18):Complete genomic instability experiment and load cells for sub-nuclear foci assay (Day4)
- 20.109(F18):Complete in silico cloning (Day1)
- 20.109(F18):Complete sub-nuclear foci assay (Day6)
- 20.109(F18):Confirm gRNA sequence (Day5)
- 20.109(F18):Construct cathode with phage-based active material (Day3)
- 20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)
- 20.109(F18):Design gRNA for CRISPRi (Day2)
- 20.109(F18):Evaluate cell loading experiment and treat cells for genomic instability experiment (Day3)
- 20.109(F18):Generate gRNA plasmid (Day3)
- 20.109(F18):Growth of phage-based active (cathode) material (Day1)
- 20.109(F18):Homework
- 20.109(F18):Image sub-nuclear foci assay
- 20.109(F18):Image sub-nuclear foci assay (Day6)
- 20.109(F18):Induce CRISPRi system (Day7)
- 20.109(F18):Journal club presentation (Day4 and 6)
- 20.109(F18):Lab tour
- 20.109(F18):Measure fermentation products (Day8)
- 20.109(F18):Module 1
- 20.109(F18):Module 2
- 20.109(F18):Module 3
- 20.109(F18):Practice statistical analysis methods and complete data analysis (Day7)
- 20.109(F18):Practice tissue culture and prepare microwell array (Day1)
- 20.109(F18):Prep notes for M3
- 20.109(F18):Prepare and treat cells for genomic instability experiment (Day3)
- 20.109(F18):Purify phage-based active (cathode) material (Day2)
- 20.109(F18):Research proposal presentation
- 20.109(F18):Visualize phage nanowires using TEM (Day4)
- 20.109(F18): Announcements
- 20.109(F18): Assignments
- 20.109(F18): Data Summary
- 20.109(F18): Fall 2018 schedule
- 20.109(F18): Mini-presentation
- 20.109(F18): Mini-report
- 20.109(F18): Prep notes for M1
- 20.109(F18): Prep notes for M2
- 20.109(F18): Research article
- 20.109(F19):Begin Western blot analysis (Day3)
- 20.109(F19):Class data
- 20.109(F19):Communication
- 20.109(F19):Complete CometChip experiment (Day5)
- 20.109(F19):Complete antibody staining for Western blot analysis (Day4)
- 20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)
- 20.109(F19):Complete in silico cloning (Day1)
- 20.109(F19):Complete in silico cloning of pdCas9 (Day1)
- 20.109(F19):Confirm gRNA sequence in pgRNA(Day5)
- 20.109(F19):Confirm gRNA sequence in pgRNA (Day5)
- 20.109(F19):Data analysis and assignment preparation (Day5)
- 20.109(F19):Design gRNA for CRISPRi (Day2)
- 20.109(F19):Design gRNA sequence for CRISPRi (Day2)
- 20.109(F19):Generate gRNA plasmid (Day3)
- 20.109(F19):Guidelines for working in the tissue culture facility
- 20.109(F19):Homework
- 20.109(F19):Image CometChip (Day6)
- 20.109(F19):Incubate with ligand and apply heat treatment for protein denaturation (Day2)
- 20.109(F19):Induce CRISPRi system (Day7)
- 20.109(F19):Journal club presentation (Day4 and 6)
- 20.109(F19):Lab tour
- 20.109(F19):Load cells into CometChip and apply treatments (Day4)
- 20.109(F19):Measure fermentation products (Day8)
- 20.109(F19):Module 1
- 20.109(F19):Module 2
- 20.109(F19):Module 3
- 20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)
- 20.109(F19):Practice statistical analysis methods and complete data analysis (Day7)
- 20.109(F19):Prepare for cellular thermal shift assay (Day1)