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- 15:34, 1 March 2021 (diff | hist) . . (+123) . . 20.109(S21):M1D4 (→Part 3:Applying our knowledge of immunity to pandemic problem solving)
- 15:23, 1 March 2021 (diff | hist) . . (+18) . . 20.109(S21):M1D4 (→Part 3:Applying our knowledge of immunity to pandemic problem solving)
- 15:22, 1 March 2021 (diff | hist) . . (+1,392) . . 20.109(S21):M1D4 (→Part 3:Applying our knowledge of immunity to pandemic problem solving)
- 15:14, 1 March 2021 (diff | hist) . . (+240) . . 20.109(S21):M1D4 (→Part 3:Applying our knowledge of immunity to pandemic problem solving)
- 15:12, 1 March 2021 (diff | hist) . . (+1,737) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 15:05, 25 February 2021 (diff | hist) . . (+41) . . 20.109(S21):Spring 2021 schedule
- 15:04, 25 February 2021 (diff | hist) . . (0) . . N File:Sp21 Mod1 lecture 3.pdf (current)
- 21:11, 23 February 2021 (diff | hist) . . (+6) . . 20.109(S21):M1D2 (→Protocols:)
- 17:33, 23 February 2021 (diff | hist) . . (-8) . . 20.109(S21):M1D2 (→Part 1: Prepare cells for cell sorting)
- 22:54, 22 February 2021 (diff | hist) . . (+42) . . 20.109(S21):Spring 2021 schedule
- 22:53, 22 February 2021 (diff | hist) . . (0) . . N File:Sp21 Mod1 lecture 2.pdf (current)
- 20:38, 20 February 2021 (diff | hist) . . (0) . . 20.109(S21):Module 1 (→Lab links: day by day) (current)
- 17:12, 18 February 2021 (diff | hist) . . (+41) . . 20.109(S21):Spring 2021 schedule
- 17:12, 18 February 2021 (diff | hist) . . (0) . . N File:Sp21 Mod1 lecture 1.pdf (current)
- 17:45, 17 February 2021 (diff | hist) . . (+21) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 17:43, 17 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 17:40, 17 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D2 (→Introduction: Enrich candidate clones from library using FACS (FACS))
- 17:39, 17 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D2 (→Introduction: Enrich candidate clones from library using FACS (FACS))
- 17:39, 17 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D2 (→Part 1: Prepare cells for cell sorting)
- 17:39, 17 February 2021 (diff | hist) . . (+123) . . 20.109(S21):M1D2 (→Part 1: Prepare cells for cell sorting)
- 12:39, 17 February 2021 (diff | hist) . . (+9) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 12:37, 17 February 2021 (diff | hist) . . (+134) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 12:34, 17 February 2021 (diff | hist) . . (+195) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 12:28, 17 February 2021 (diff | hist) . . (+329) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 12:21, 17 February 2021 (diff | hist) . . (+382) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 18:59, 16 February 2021 (diff | hist) . . (+122) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 18:57, 16 February 2021 (diff | hist) . . (+69) . . 20.109(S21):M1D2 (→Introduction: Enrich candidate clones from library using FACS (FACS))
- 18:55, 16 February 2021 (diff | hist) . . (+156) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 17:54, 16 February 2021 (diff | hist) . . (+62) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 17:52, 16 February 2021 (diff | hist) . . (+272) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 12:15, 16 February 2021 (diff | hist) . . (+203) . . 20.109(S21):M1D1 (→Part 3: Gel purify PCR products)
- 01:08, 16 February 2021 (diff | hist) . . (-17) . . 20.109(S21):M1D7 (→Part 2: Plot titration binding curves via Matlab)
- 01:07, 16 February 2021 (diff | hist) . . (+1) . . 20.109(S21):M1D7 (→Protocols)
- 01:07, 16 February 2021 (diff | hist) . . (+116) . . 20.109(S21):M1D7 (→Part 1: Analyze flow cytometry scatter plots using FlowJo and generate MFI table)
- 01:06, 16 February 2021 (diff | hist) . . (+118) . . 20.109(S21):M1D7 (→Part 2: Plot titration binding curves in excel)
- 01:05, 16 February 2021 (diff | hist) . . (+117) . . 20.109(S21):M1D6 (→Part 2: Accuri Flow Cytometry of Stained Yeast Samples)
- 01:05, 16 February 2021 (diff | hist) . . (+20) . . 20.109(S21):M1D6 (→Part 1: Prepare Titration Lysozyme with scFv Clone on Yeast)
- 00:49, 16 February 2021 (diff | hist) . . (+20) . . 20.109(S21):M1D5 (→Part 2: Align scFv sequences)
- 00:49, 16 February 2021 (diff | hist) . . (+3) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 00:48, 16 February 2021 (diff | hist) . . (-64) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 00:47, 16 February 2021 (diff | hist) . . (+347) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 00:39, 16 February 2021 (diff | hist) . . (+417) . . 20.109(S21):M1D4 (→Part 1: Harvest plasmid (Miniprep) from E. coli)
- 00:31, 16 February 2021 (diff | hist) . . (+59) . . 20.109(S21):M1D4 (→Part 1: Harvest plasmid (Miniprep) from E. coli)
- 14:20, 14 February 2021 (diff | hist) . . (-111) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 14:19, 14 February 2021 (diff | hist) . . (+4) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 14:19, 14 February 2021 (diff | hist) . . (-3) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 14:18, 14 February 2021 (diff | hist) . . (-1) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 14:18, 14 February 2021 (diff | hist) . . (-149) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 14:12, 14 February 2021 (diff | hist) . . (-7) . . 20.109(S21):M1D4 (→Introduction:Purify and sequence clone plasmids)
- 14:12, 14 February 2021 (diff | hist) . . (+127) . . 20.109(S21):M1D4 (→Introduction:Purify and sequence clone plasmids)
- 14:09, 14 February 2021 (diff | hist) . . (+13) . . 20.109(S21):M1D4 (→Introduction:Purify and sequence clone plasmids)
- 14:08, 14 February 2021 (diff | hist) . . (+12) . . 20.109(S21):M1D4 (→Introduction:Purify and sequence clone plasmids)
- 14:07, 14 February 2021 (diff | hist) . . (+139) . . 20.109(S21):M1D4 (→Introduction:Purify and sequence clone plasmids)
- 13:51, 14 February 2021 (diff | hist) . . (+267) . . 20.109(S21):Homework (→Due M1D3)
- 21:07, 13 February 2021 (diff | hist) . . (+132) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 21:01, 13 February 2021 (diff | hist) . . (+5) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:58, 13 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:58, 13 February 2021 (diff | hist) . . (+2) . . 20.109(S21):M1D3 (→Part 3: Transform plasmid from yeast into E. coli)
- 20:57, 13 February 2021 (diff | hist) . . (+139) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:55, 13 February 2021 (diff | hist) . . (0) . . N File:DNAmeasurement2.jpg (current)
- 20:54, 13 February 2021 (diff | hist) . . (0) . . N File:DNAmeasurement1.jpg (current)
- 20:54, 13 February 2021 (diff | hist) . . (+217) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:49, 13 February 2021 (diff | hist) . . (-5) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:48, 13 February 2021 (diff | hist) . . (+744) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:39, 13 February 2021 (diff | hist) . . (-1) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:38, 13 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:38, 13 February 2021 (diff | hist) . . (+2) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:37, 13 February 2021 (diff | hist) . . (-2) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:37, 13 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:36, 13 February 2021 (diff | hist) . . (-3) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:36, 13 February 2021 (diff | hist) . . (0) . . N File:DNASpec3.jpg (current)
- 20:35, 13 February 2021 (diff | hist) . . (0) . . N File:DNAspec2.jpg (current)
- 20:35, 13 February 2021 (diff | hist) . . (0) . . N File:DNAspec1.jpg (current)
- 20:35, 13 February 2021 (diff | hist) . . (+25) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:34, 13 February 2021 (diff | hist) . . (-1) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:34, 13 February 2021 (diff | hist) . . (+201) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:27, 13 February 2021 (diff | hist) . . (+58) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 20:26, 13 February 2021 (diff | hist) . . (+77) . . 20.109(S21):M1D3 (→Part 3: Transform plasmid from yeast into E. coli)
- 20:24, 13 February 2021 (diff | hist) . . (+147) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 19:56, 13 February 2021 (diff | hist) . . (+1,151) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 19:33, 13 February 2021 (diff | hist) . . (-324) . . 20.109(S21):M1D2 (→Part 1: Prepare cells for cell sorting)
- 16:53, 13 February 2021 (diff | hist) . . (+324) . . 20.109(S21):M1D2 (→Part 1: Prepare cells for cell sorting)
- 16:48, 13 February 2021 (diff | hist) . . (+82) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:39, 13 February 2021 (diff | hist) . . (+276) . . 20.109(S21):M1D2 (→Part 3: Participate in group paper discussion)
- 15:20, 13 February 2021 (diff | hist) . . (+1) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 15:20, 13 February 2021 (diff | hist) . . (+2) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 15:20, 13 February 2021 (diff | hist) . . (+3) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 15:20, 13 February 2021 (diff | hist) . . (+5) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 15:20, 13 February 2021 (diff | hist) . . (+7) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 15:20, 13 February 2021 (diff | hist) . . (+5) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 15:19, 13 February 2021 (diff | hist) . . (+6) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 15:19, 13 February 2021 (diff | hist) . . (-28) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 15:18, 13 February 2021 (diff | hist) . . (-6) . . 20.109(S21):M1D6 (→Protocols)
- 15:18, 13 February 2021 (diff | hist) . . (+6) . . 20.109(S21):M1D6 (→Part 1: Prepare Titration Lysozyme with scFv Clone on Yeast)
- 15:18, 13 February 2021 (diff | hist) . . (+97) . . 20.109(S21):M1D6 (→Part 1: Prepare Titration Lysozyme with scFv Clone on Yeast)
- 15:17, 13 February 2021 (diff | hist) . . (+1) . . 20.109(S21):M1D5 (→Part 2: Align scFv sequences)
- 15:16, 13 February 2021 (diff | hist) . . (+96) . . 20.109(S21):M1D5 (→Part 2: Align scFv sequences)
- 15:15, 13 February 2021 (diff | hist) . . (+97) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 15:15, 13 February 2021 (diff | hist) . . (+97) . . 20.109(S21):M1D4 (→Part 1: Harvest plasmid (Miniprep) from E. coli)
- 15:15, 13 February 2021 (diff | hist) . . (+115) . . 20.109(S21):M1D3 (→Part 3: Transform plasmid from yeast into E. coli)
- 15:09, 13 February 2021 (diff | hist) . . (+285) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:56, 13 February 2021 (diff | hist) . . (-4) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:55, 13 February 2021 (diff | hist) . . (+21) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:54, 13 February 2021 (diff | hist) . . (+88) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:53, 13 February 2021 (diff | hist) . . (+54) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:47, 13 February 2021 (diff | hist) . . (+135) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:45, 13 February 2021 (diff | hist) . . (+2) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:45, 13 February 2021 (diff | hist) . . (+113) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:42, 13 February 2021 (diff | hist) . . (+37) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:41, 13 February 2021 (diff | hist) . . (+166) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:38, 13 February 2021 (diff | hist) . . (+1) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:37, 13 February 2021 (diff | hist) . . (+2) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:37, 13 February 2021 (diff | hist) . . (+76) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:33, 13 February 2021 (diff | hist) . . (+154) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 14:27, 13 February 2021 (diff | hist) . . (+37) . . 20.109(S21):M1D1 (→Part 5: Transform DNA into yeast)
- 14:26, 13 February 2021 (diff | hist) . . (+161) . . 20.109(S21):M1D1 (→Restriction enzyme digest of pCTcon2 backbone)
- 14:23, 13 February 2021 (diff | hist) . . (+192) . . 20.109(S21):M1D1 (→Restriction enzyme digest of pCTcon2 backbone)
- 14:21, 13 February 2021 (diff | hist) . . (+6) . . 20.109(S21):M1D1 (→Restriction enzyme digest of pCTcon2 backbone)
- 14:20, 13 February 2021 (diff | hist) . . (+19) . . 20.109(S21):M1D1 (→Restriction enzyme digests)
- 14:15, 13 February 2021 (diff | hist) . . (+8) . . 20.109(S21):M1D1 (→Part 4: Prepare error-prone PCR products and backbone for yeast transformation)
- 14:14, 13 February 2021 (diff | hist) . . (+1) . . 20.109(S21):M1D1 (→Part 3: Gel purify PCR products)
- 14:13, 13 February 2021 (diff | hist) . . (+109) . . 20.109(S21):M1D1 (→Part 3: Gel purify PCR products)
- 14:11, 13 February 2021 (diff | hist) . . (+6) . . 20.109(S21):M1D1 (→Part 2: Mutagenesis of scFv clone using Error-prone PCR)
- 14:09, 13 February 2021 (diff | hist) . . (+36) . . 20.109(S21):M1D1 (→Part 1: Investigate our parental scFv clone: plasmid Lyso_scFv_6)
- 20:38, 12 February 2021 (diff | hist) . . (0) . . N File:Sp21 library 5nM.JPG (current)
- 20:38, 12 February 2021 (diff | hist) . . (0) . . N File:Sp21 negControl 488.JPG (current)
- 20:38, 12 February 2021 (diff | hist) . . (0) . . N File:Sp21 negControl 647.JPG (current)
- 20:38, 12 February 2021 (diff | hist) . . (0) . . N File:Sp21 positive control.JPG (current)
- 20:37, 12 February 2021 (diff | hist) . . (0) . . N File:Sp21 yeast no stain.JPG (current)
- 20:19, 12 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 20:18, 12 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 20:18, 12 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 20:18, 12 February 2021 (diff | hist) . . (+6) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 19:01, 12 February 2021 (diff | hist) . . (+8) . . 20.109(S21):M1D7 (→Protocols)
- 19:00, 12 February 2021 (diff | hist) . . (+16) . . 20.109(S21):M1D5 (→Part 2: Align scFv sequences)
- 18:59, 12 February 2021 (diff | hist) . . (+6) . . 20.109(S21):M1D4 (→Part 2: Sequencing scFv clones)
- 18:59, 12 February 2021 (diff | hist) . . (+3) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 18:58, 12 February 2021 (diff | hist) . . (+3) . . 20.109(S21):M1D2 (→Part 1: Prepare cells for cell sorting)
- 18:58, 12 February 2021 (diff | hist) . . (+6) . . 20.109(S21):M1D2 (→Introduction: Enrich candidate clones from library using FACS (FACS))
- 18:57, 12 February 2021 (diff | hist) . . (+408) . . 20.109(S21):M1D1 (→Part 3: Gel purify PCR products)
- 18:50, 12 February 2021 (diff | hist) . . (+179) . . 20.109(S21):M1D1 (→Restriction enzyme digests)
- 18:05, 12 February 2021 (diff | hist) . . (0) . . N File:Sp21 pCTCON2 gel.jpg (current)
- 17:02, 12 February 2021 (diff | hist) . . (+5) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 17:02, 12 February 2021 (diff | hist) . . (-27) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 17:00, 12 February 2021 (diff | hist) . . (+4) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:59, 12 February 2021 (diff | hist) . . (+8) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:59, 12 February 2021 (diff | hist) . . (+5) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:58, 12 February 2021 (diff | hist) . . (+1) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:57, 12 February 2021 (diff | hist) . . (-22) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:57, 12 February 2021 (diff | hist) . . (+15) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:56, 12 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:53, 12 February 2021 (diff | hist) . . (+10) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:53, 12 February 2021 (diff | hist) . . (+127) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:52, 12 February 2021 (diff | hist) . . (0) . . N File:Sp21 AriaFACS.jpeg (current)
- 16:52, 12 February 2021 (diff | hist) . . (0) . . N File:Sp21 Ariainside.jpeg (current)
- 16:31, 12 February 2021 (diff | hist) . . (-1) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:31, 12 February 2021 (diff | hist) . . (+43) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting (FACS))
- 16:29, 12 February 2021 (diff | hist) . . (+284) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting)
- 16:25, 12 February 2021 (diff | hist) . . (+268) . . 20.109(S21):M1D2 (→Part 1: Prepare cells for cell sorting)
- 16:08, 12 February 2021 (diff | hist) . . (+2) . . 20.109(S21):M1D2 (→Protocols:)
- 19:40, 11 February 2021 (diff | hist) . . (+4) . . 20.109(S21):Homework (→Due M1D3)
- 19:37, 11 February 2021 (diff | hist) . . (+30) . . 20.109(S21):M1D6 (→Reagents list)
- 19:35, 11 February 2021 (diff | hist) . . (-8) . . 20.109(S21):M1D2 (→Reagents)
- 19:29, 11 February 2021 (diff | hist) . . (+222) . . 20.109(S21):M1D2 (→Reagents)
- 19:15, 11 February 2021 (diff | hist) . . (+46) . . 20.109(S21):M1D2 (→Protocols:)
- 19:03, 11 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D2 (→Reagents)
- 19:03, 11 February 2021 (diff | hist) . . (+7) . . 20.109(S21):M1D2 (→Reagents)
- 19:03, 11 February 2021 (diff | hist) . . (+3) . . 20.109(S21):M1D2 (→Protocols:)
- 18:44, 11 February 2021 (diff | hist) . . (-8) . . 20.109(S21):Homework (→Due M1D5)
- 18:44, 11 February 2021 (diff | hist) . . (0) . . N File:Homework M1D5 result interpretation.jpg (current)
- 18:35, 11 February 2021 (diff | hist) . . (+2) . . 20.109(S21):Homework (→Due M1D5)
- 18:32, 11 February 2021 (diff | hist) . . (+109) . . 20.109(S21):M1D1 (→Part 4: Prepare error-prone PCR products and backbone for yeast transformation)
- 18:30, 11 February 2021 (diff | hist) . . (+70) . . 20.109(S21):M1D1 (→Part 4: Prepare error-prone PCR products and backbone for yeast transformation)
- 18:28, 11 February 2021 (diff | hist) . . (-1) . . 20.109(S21):M1D1 (→Part 4: Prepare error-prone PCR products and backbone for yeast transformation)
- 18:27, 11 February 2021 (diff | hist) . . (+293) . . 20.109(S21):M1D1 (→Part 4: Prepare error-prone PCR products and backbone for yeast transformation)
- 18:24, 11 February 2021 (diff | hist) . . (+4) . . 20.109(S21):M1D1 (→Part 2: Mutagenesis of scFv clone using Error-prone PCR)
- 18:23, 11 February 2021 (diff | hist) . . (+5) . . 20.109(S21):M1D1 (→Part 1: Investigate our parental scFv clone: plasmid Lyso_scFv_6)
- 18:22, 11 February 2021 (diff | hist) . . (-3) . . 20.109(S21):M1D1 (→Part 1: Investigate our parental scFv clone: plasmid Lyso_scFv_6)
- 18:22, 11 February 2021 (diff | hist) . . (+5) . . 20.109(S21):M1D1 (→Part 1: Investigate our parental scFv clone: plasmid Lyso_scFv_6)
- 18:21, 11 February 2021 (diff | hist) . . (0) . . N File:Sp21 Lyso scFv 6.jpg (current)
- 18:19, 11 February 2021 (diff | hist) . . (0) . . File:PCTcon2.dna (Leslie McClain uploaded a new version of "File:PCTcon2.dna") (current)
- 18:19, 11 February 2021 (diff | hist) . . (0) . . N File:Sp21 Lyso scFv 6.dna (current)
- 18:00, 11 February 2021 (diff | hist) . . (-1) . . 20.109(S21):M1D1 (→Part 1: Investigate our parental scFv clone: plasmid ADI-31375)
- 03:18, 11 February 2021 (diff | hist) . . (+1) . . 20.109(S21):Homework (→Due M1D6)
- 03:17, 11 February 2021 (diff | hist) . . (+116) . . 20.109(S21):Homework (→Due M1D6)
- 03:14, 11 February 2021 (diff | hist) . . (+18) . . 20.109(S21):Module 1 (→Engineering Antibodies Using Yeast Display)
- 03:11, 11 February 2021 (diff | hist) . . (+8) . . 20.109(S21):Data Summary (→Implications and Future Work: potential topics)
- 03:08, 11 February 2021 (diff | hist) . . (+154) . . 20.109(S21):Homework (→Due M1D7)
- 02:55, 11 February 2021 (diff | hist) . . (+204) . . 20.109(S21):Homework (→Due M1D7)
- 02:48, 11 February 2021 (diff | hist) . . (+8) . . 20.109(S21):Homework (→Due M1D6)
- 02:48, 11 February 2021 (diff | hist) . . (0) . . 20.109(S21):Homework (→Due M1D6)
- 02:47, 11 February 2021 (diff | hist) . . (+15) . . 20.109(S21):Homework (→Due M1D6)
- 02:47, 11 February 2021 (diff | hist) . . (-10) . . 20.109(S21):Homework (→Due M1D6)
- 02:43, 11 February 2021 (diff | hist) . . (+43) . . 20.109(S21):Homework (→Due M1D5)
- 02:41, 11 February 2021 (diff | hist) . . (0) . . 20.109(S21):Homework (→Due M1D4)
- 02:30, 11 February 2021 (diff | hist) . . (-44) . . 20.109(S21):Homework (→Due M1D3)
- 02:28, 11 February 2021 (diff | hist) . . (-2) . . 20.109(S21):Homework (→Due M1D2)
- 02:22, 11 February 2021 (diff | hist) . . (+30) . . 20.109(S21):M1D2 (→Introduction: Enrich candidate clones from library using FACS (FACS))
- 01:07, 11 February 2021 (diff | hist) . . (+484) . . 20.109(S21):M1D1 (→Restriction enzyme digests)
- 00:50, 11 February 2021 (diff | hist) . . (-13) . . 20.109(S21):M1D1 (→Part 4: Prepare error-prone PCR products and backbone for yeast transformation)
- 00:49, 11 February 2021 (diff | hist) . . (+251) . . 20.109(S21):M1D1 (→Part 4: Prepare error-prone PCR products and backbone for yeast transformation)
- 00:42, 11 February 2021 (diff | hist) . . (+80) . . 20.109(S21):M1D1 (→Part 4: Prepare error-prone PCR products and backbone for yeast transformation)
- 00:25, 11 February 2021 (diff | hist) . . (0) . . File:Ab31375 Map.jpg (Leslie McClain uploaded a new version of "File:Ab31375 Map.jpg") (current)
- 20:48, 10 February 2021 (diff | hist) . . (0) . . File:Sp21 PCR2.jpg (Leslie McClain uploaded a new version of "File:Sp21 PCR2.jpg") (current)
- 15:42, 9 February 2021 (diff | hist) . . (+54) . . 20.109(S21):Announcements (→Office hours for Spring 2021)
- 15:24, 9 February 2021 (diff | hist) . . (+17) . . 20.109(S21):Module 1 (→Engineering Antibodies Using Yeast Display)
- 15:19, 9 February 2021 (diff | hist) . . (-1) . . 20.109(S21):Module 1 (→References)
- 15:19, 9 February 2021 (diff | hist) . . (+14) . . 20.109(S21):Module 1 (→References)
- 15:18, 9 February 2021 (diff | hist) . . (-1) . . 20.109(S21):Module 1 (→References)
- 15:17, 9 February 2021 (diff | hist) . . (-2) . . 20.109(S21):Module 1 (→References)
- 15:17, 9 February 2021 (diff | hist) . . (-7) . . 20.109(S21):Module 1 (→References)
- 15:16, 9 February 2021 (diff | hist) . . (+249) . . 20.109(S21):Module 1 (→References)
- 02:37, 9 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D1 (→Part 3: Gel purify PCR products)
- 02:37, 9 February 2021 (diff | hist) . . (+64) . . 20.109(S21):M1D1 (→Part 3: Gel purify PCR products)
- 02:36, 9 February 2021 (diff | hist) . . (0) . . N File:Sp21 pcrgelimage.jpg (current)
- 02:35, 9 February 2021 (diff | hist) . . (0) . . N File:NEB 1kbDNAladder.jpg (current)
- 02:34, 9 February 2021 (diff | hist) . . (+1) . . 20.109(S21):M1D1 (→Part 1: Investigate our parental scFv clone: plasmid ADI-31375)
- 02:33, 9 February 2021 (diff | hist) . . (+79) . . 20.109(S21):M1D1 (→Part 3: Gel purify PCR products)
- 02:20, 9 February 2021 (diff | hist) . . (-14) . . 20.109(S21):M1D1 (→Part 3: Gel purify PCR products)
- 01:53, 9 February 2021 (diff | hist) . . (-1,200) . . 20.109(S21):M1D7 (→Part 2: Plot titration binding curves in excel)
- 01:04, 9 February 2021 (diff | hist) . . (+97) . . 20.109(S21):M1D3 (→Part 3: Transform plasmid from yeast into E. coli)
- 01:04, 9 February 2021 (diff | hist) . . (+97) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to measure DNA concentration)
- 01:03, 9 February 2021 (diff | hist) . . (+20) . . 20.109(S21):M1D3 (→Part2: Using a spectrophotometer to meause DNA concentration)
- 00:55, 9 February 2021 (diff | hist) . . (-10) . . 20.109(S21):M1D3 (→Part 1: Harvest plasmid DNA from yeast)
- 00:54, 9 February 2021 (diff | hist) . . (+13) . . 20.109(S21):M1D3 (→Part 1: Harvest plasmid DNA from yeast)
- 00:51, 9 February 2021 (diff | hist) . . (+35) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting)
- 22:48, 8 February 2021 (diff | hist) . . (+144) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting)
- 22:46, 8 February 2021 (diff | hist) . . (+395) . . 20.109(S21):M1D2 (→Part 2: Fluorescence Activated Cell Sorting)
- 22:32, 8 February 2021 (diff | hist) . . (+33) . . 20.109(S21):M1D2 (→Part 1: Prepare cells for cell sorting)
- 22:19, 8 February 2021 (diff | hist) . . (+13) . . 20.109(S21):M1D2 (→Part 1: Prepare cells for cell sorting)
- 22:19, 8 February 2021 (diff | hist) . . (+385) . . 20.109(S21):M1D2 (→Part 1: Prepare cells for cell sorting)
- 22:06, 8 February 2021 (diff | hist) . . (+874) . . 20.109(S21):M1D2 (→Protocols:)
- 21:38, 8 February 2021 (diff | hist) . . (-8) . . 20.109(S21):M1D2 (→Introduction: Enrich candidate clones from library using FACS (FACS))
- 21:32, 8 February 2021 (diff | hist) . . (+4) . . 20.109(S21):M1D1 (→Introduction: Generate single-chain antibody fragment (scFv) library)
- 21:31, 8 February 2021 (diff | hist) . . (+4) . . 20.109(S21):M1D1 (→Introduction: Generate single-chain antibody fragment (scFv) library)
- 19:47, 8 February 2021 (diff | hist) . . (+60) . . 20.109(S21):M1D1 (→Part 2: Mutagenesis of scFv clone using Error-prone PCR)
- 19:47, 8 February 2021 (diff | hist) . . (+11) . . 20.109(S21):M1D1 (→Part 2: Mutagenesis of scFv clone using Error-prone PCR)
- 19:44, 8 February 2021 (diff | hist) . . (+35) . . 20.109(S21):M1D1 (→Part 2: Mutagenesis of scFv clone using Error-prone PCR)
- 19:42, 8 February 2021 (diff | hist) . . (+6) . . 20.109(S21):M1D1 (→Part 1: Investigate our parental scFv clone: plasmid ADI-31375)
- 19:42, 8 February 2021 (diff | hist) . . (-6) . . 20.109(S21):M1D1 (→Part 1: Investigate our parental scFv clone: plasmid ADI-31375)
- 19:40, 8 February 2021 (diff | hist) . . (+35) . . 20.109(S21):M1D1 (→Introduction: Generate single-chain antibody fragment (scFv) library)
- 15:58, 5 February 2021 (diff | hist) . . (0) . . 20.109(S21):M1D1 (→Part 2: Mutagenesis of scFv clone using Error-prone PCR)
- 15:57, 5 February 2021 (diff | hist) . . (+118) . . 20.109(S21):M1D1 (→Part 2: Mutagenesis of scFv clone using Error-prone PCR)
- 02:59, 5 February 2021 (diff | hist) . . (-1) . . 20.109(S21):Homework (→Due M1D2)
- 02:59, 5 February 2021 (diff | hist) . . (-6) . . 20.109(S21):Homework (→Due M1D2)
- 02:59, 5 February 2021 (diff | hist) . . (-47) . . 20.109(S21):Homework (→Due M1D2)
- 02:58, 5 February 2021 (diff | hist) . . (+178) . . 20.109(S21):Homework (→Due M1D2)
- 02:56, 5 February 2021 (diff | hist) . . (+1) . . 20.109(S21):Module 1 (→References)
- 02:55, 5 February 2021 (diff | hist) . . (+21) . . 20.109(S21):Module 1 (→References)
- 02:54, 5 February 2021 (diff | hist) . . (+1) . . 20.109(S21):Module 1 (→References)
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