Difference between revisions of "Assignment 8 Overview"

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#** Refer to schematics and diagrams in the lab manual instead of copying them into your report. Use reference designators (such as R7, C2) to refer to call out particular components in schematic diagrams.
 
#** Refer to schematics and diagrams in the lab manual instead of copying them into your report. Use reference designators (such as R7, C2) to refer to call out particular components in schematic diagrams.
 
#* Why not include a nice snapshot or two of the instrument? So lovely.
 
#* Why not include a nice snapshot or two of the instrument? So lovely.
 +
#* Report the signal-to-noise ratio of your instrument.
 +
#* Document the procedure used to gather your data with fluorescein or beater DNA:
 +
#** Refer to procedures in the lab manual. Describe any changes you made.
 +
#** Report instrument settings for each trial, including control software parameters.
 +
#* Plot all of your raw data, fluorescence vs. block temperature, on the smallest number of axes that clearly conveys the dataset. Include only data generated by your own group.
 
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Revision as of 18:19, 1 November 2017

20.309: Biological Instrumentation and Measurement

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Assignment details

This assignment has 2 parts:

  1. Part 1: Let's check that you're comfortable with convolution and the lock-in amplifier design;
  2. Part 2: Modify your instrument to include a lock-in amplifier and temperature control.

Submit your work on Stellar in a single PDF file with the naming convention <Lastname><Firstname>Assignment8.pdf.


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Here is a checklist of all things you have to turn in:

  1. answers to all questions in Assignment 8, Part 1;
  2. a technical subsection of a lab report on your apparatus ameliorated with lock-in amplifier and temperature control:
    • Document your instrument design.
      • Describe your apparatus with sufficient detail for another person to replicate your work. Assume the reader is familiar with the concepts of 20.309 and has access to course materials.
      • Detail your electronic and optical subsystems. Include component values, gain values, cutoff frequencies, lens focal lengths, and relevant distances.
      • It is not necessary to document construction details, unless you built an instrument that was significantly different than the lab manual suggested.
      • Refer to schematics and diagrams in the lab manual instead of copying them into your report. Use reference designators (such as R7, C2) to refer to call out particular components in schematic diagrams.
    • Why not include a nice snapshot or two of the instrument? So lovely.
    • Report the signal-to-noise ratio of your instrument.
    • Document the procedure used to gather your data with fluorescein or beater DNA:
      • Refer to procedures in the lab manual. Describe any changes you made.
      • Report instrument settings for each trial, including control software parameters.
    • Plot all of your raw data, fluorescence vs. block temperature, on the smallest number of axes that clearly conveys the dataset. Include only data generated by your own group.


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Background reading

Code examples and simulations

Reference materials

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