Difference between revisions of "20.109(S24):Spring 2024 schedule"

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| R/F Mar 21/22  
 
| R/F Mar 21/22  
 
| [http://be.mit.edu/directory/angela-belcher AMB] <br> [[Media:Sp24 L3 AMB.pdf | Lecture slides]]
 
| [http://be.mit.edu/directory/angela-belcher AMB] <br> [[Media:Sp24 L3 AMB.pdf | Lecture slides]]
| [[20.109(S24):M2D4 |Align sequencing and prepare for Journal Article presentations]] <br>  
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| [[20.109(S24):M2D4 |Align sequencing and prepare for Journal Article presentations]] <br> [[Media:Sp24 TR JADiscussion bcm.pdf |TR prelab slides]] <br> [[Media:Sp24 WF JADiscussion bcm.pdf | WF prelab]]
 
| <font color= #3bc2b6 >'''Laboratory quiz'''</font color> <br> [[20.109(S24):Homework#Due_M2D4|Homework due]]  
 
| <font color= #3bc2b6 >'''Laboratory quiz'''</font color> <br> [[20.109(S24):Homework#Due_M2D4|Homework due]]  
 
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Revision as of 17:40, 22 March 2024

20.109(S24): Laboratory Fundamentals of Biological Engineering

Sp24 banner image.png

Spring 2024 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Protein engineering        M3: Project design       


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 4-237
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322

ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).

MODULE DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Feb 6/7 NLL
Lecture slides
Orientation and laboratory tour
EHS slides
TR prelab slides
WF prelab slides
M1D1 R/F Feb 8/9 AK
Lecture slides
Complete in-silico cloning of protein expression vector
TR prelab slides
WF prelab slides
Orientation quiz
Homework due
M1D2 T/W Feb 13/14 AK
Purify expressed protein
TR prelab slides & Protein purification video
WF prelab slides
Homework due
M1D3 R/F Feb 15/16 AK
Lecture slides
Assess purity and concentration of expressed protein
TR prelab slides
WF prelab slides
Homework due
T/W Feb 20/21 President's Day holiday
M1D4 R/F Feb 22/23 AK
Lecture slides
Review results of small molecule microarray (SMM) screen
TR prelab slides
WF prelab slides
Laboratory quiz
Homework due
M1D5 T/W Feb 27/28 AK
Lecture slides
Setup differential scanning flourimetry (DSF) experiment
TR prelab slides
WF prelab slides
Homework due
M1D6 R/F Feb/Mar 29/1 AK
Lecture slides
Prepare cells for electromobility shift assay (EMSA)
TR prelab slides
WF prelab slides
Homework due
Research talk due Mon, Mar 4 at 10pm
M1D7 T/W Mar 5/6 AK
Lecture slides
Complete EMSA experiment
TR prelab slides
WF prelab slides
Homework due
M1D8 R/F Mar 7/8 BE Comm Lab
Evaluate experimental results
TR & WF prelab slides
Laboratory quiz
Homework due
M2D1 T/W Mar 12/13 BCM
Lecture slides
Determine peptide design strategy
TR prelab slides
WF prelab slides
Homework due
M2D2 R/F Mar 14/15 AMB
Lecture slides
Clone cell surface peptide display plasmid
TR prelab slides
WF prelab slides
Homework due
Data Summary draft due Sat, Mar 16 at 10 pm
Blog post due Mon, Mar 18 at 10 pm
M2D3 T/W Mar 19/20 AMB
Lecture slides
Sequence clones and transform into yeast
TR prelab slides
WF prelab slides
Homework due
M2D4 R/F Mar 21/22 AMB
Lecture slides
Align sequencing and prepare for Journal Article presentations
TR prelab slides
WF prelab
Laboratory quiz
Homework due
T/W Mar 26/27 - R/F Mar 28/29 Spring Break Data Summary revision due Mon, Mar 25 at 10 pm
T/W Apr 2/3 Journal article presentations
R/F Apr 4/5 Journal article presentations Blog post due Sat, Apr 6 at 10 pm
M2D5 T/W Apr 9/10 AMB
Perform flow cytometry and harvest cells to test cadmium sequestration
Homework due
M2D6 R/F Apr 11/12 AMB
Quantify cadmium removal from media
Homework due
T/W Apr 16/17 Patriots' Day holiday
M2D7 R/F Apr 18/19 BCM
Visualize cadmium sequestration and assess quality of cadmium sulfide production
Homework due
M2D8 T/W Apr 23/24 BCM
Complete data analysis and organize Research Article figures
Laboratory quiz
Homework due
M3D1 R/F Apr 25/26 AMB
Brainstorm ideas for Research proposal presentation
Homework due
M3D2 T/W Apr/May 30/1 BE Comm Lab Pitch research proposal presentation ideas
Research article due Mon, Apr 29 at 10 pm
Blog post due Tue, Apr 30 at 10 pm
M3D3 R/F May 2/3 NLL
BCM
Develop ideas for Research proposal presentation
Homework due
M3D4 T/W May 7/8 BCM
Participate in Research proposal peer reviews
Homework due
R/F May 9/10 Research proposal presentations Blog post due Sat, May 11 at 10 pm
T May 14 Celebration lunch!