20.109(S23):M2D7

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20.109(S23): Laboratory Fundamentals of Biological Engineering

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Spring 2023 schedule        FYI        Assignments        Homework        Class data        Communication        Accessibility

       M1: Drug discovery        M2: Protein engineering        M3: Project design       

Introduction

Cell viability assay
The BacTiter-Glo Microbial Cell Viability Assay is a method for quantifying the number of viable cells based on measuring the amount of ATP present. ATP is a proxy for the presence of metabolically active (alive) cells. In this assay, the cells are lysed and ATP is released from the active cells. In a reaction catalyzed by a propriety luciferase enzyme, luciferin, ATP, and oxygen result in oxyluciferin, AMP, PPi, carbon dioxide, and light. The light product is then measured using a luminometer.

Protocols

Part 1: Analyze ICP-OES data

In your laboratory notebook, complete the following:

  • What

Part 2: Perform metal tolerance experiment

Reagents list

  • WST-8 solution (Abcam)

Navigation links

Next day: Complete data analysis and organize Research article figures

Previous day: Analyze ICP-OES data and examine yeast tolerance to metal
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