Difference between revisions of "20.109(S23):M2D7"
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==Introduction== | ==Introduction== | ||
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| + | '''Cell viability assay'''<br> | ||
| + | The BacTiter-Glo Microbial Cell Viability Assay is a method for quantifying the number of viable cells based on measuring the amount of ATP present. ATP is a proxy for the presence of metabolically active (alive) cells. In this assay, the cells are lysed and ATP is released from the active cells. In a reaction catalyzed by a propriety luciferase enzyme, luciferin, ATP, and oxygen result in oxyluciferin, AMP, PP<sub>i</sub>, carbon dioxide, and light. The light product is then measured using a luminometer. | ||
==Protocols== | ==Protocols== | ||
Revision as of 22:59, 27 February 2023
Contents
Introduction
Cell viability assay
The BacTiter-Glo Microbial Cell Viability Assay is a method for quantifying the number of viable cells based on measuring the amount of ATP present. ATP is a proxy for the presence of metabolically active (alive) cells. In this assay, the cells are lysed and ATP is released from the active cells. In a reaction catalyzed by a propriety luciferase enzyme, luciferin, ATP, and oxygen result in oxyluciferin, AMP, PPi, carbon dioxide, and light. The light product is then measured using a luminometer.
Protocols
Part 1: Analyze ICP-OES data
In your laboratory notebook, complete the following:
- What
Part 2: Perform metal tolerance experiment
Reagents list
- WST-8 solution (Abcam)
Next day: Complete data analysis and organize Research article figures
