Difference between revisions of "20.109(S20):Examine qPCR results and complete data analysis (Day8)"

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(→‎Part 1: Analyze RNA-seq data)
(→‎Part 1: Analyze RNA-seq data)
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Today you will complete your analysis of the RNA-seq data sets!   
 
Today you will complete your analysis of the RNA-seq data sets!   
  
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Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel.  In this exercise, compare the gene expression profiles of DLD-1 and A549 to identify similarities and / or differences in the transcriptome that exist when cells are treated with etoposide.
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Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel.  In this exercise, compare the transcription profiles of DLD-1 and A549 to identify similarities and / or differences in gene expression that exist when cells are treated with etoposide.
  
 
==Navigation links==
 
==Navigation links==
 
Next day: [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br>
 
Next day: [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br>
 
Previous day: [[20.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7) | Review qPCR experiment and complete statistical analysis]]<br>
 
Previous day: [[20.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7) | Review qPCR experiment and complete statistical analysis]]<br>

Revision as of 17:09, 18 March 2020

20.109(S20): Laboratory Fundamentals of Biological Engineering

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Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Introduction

Protocols

Part 1: Analyze RNA-seq data

Today you will complete your analysis of the RNA-seq data sets!

Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, compare the transcription profiles of DLD-1 and A549 to identify similarities and / or differences in gene expression that exist when cells are treated with etoposide.

Navigation links

Next day: Enrich candidate clones using fluorescence-activated cell sorter (FACS)

Previous day: Review qPCR experiment and complete statistical analysis
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