Difference between revisions of "20.109(S18): Spring 2018 schedule"
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| T/W Feb 6/7 | | T/W Feb 6/7 | ||
| − | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Sp18 Orientation lecture.pptx|Orientation | + | | [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Sp18 Orientation lecture.pptx|Orientation Lecture]] |
| [[20.109(S18):Laboratory tour | Orientation and laboratory tour]]<br>[[Media:Sp18 Orientation NLL marked.pdf| T/R Prelab]]<br>[[Media:Sp18 Orientation JB marked.pdf| W/F Prelab]] | | [[20.109(S18):Laboratory tour | Orientation and laboratory tour]]<br>[[Media:Sp18 Orientation NLL marked.pdf| T/R Prelab]]<br>[[Media:Sp18 Orientation JB marked.pdf| W/F Prelab]] | ||
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| 1 | | 1 | ||
| R/F Feb 8/9 | | R/F Feb 8/9 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:Sp18 20109 M1L1.pdf| M1D1 | + | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:Sp18 20109 M1L1.pdf| M1D1 Lecture]] |
| [[20.109(S18):In silico cloning and confirmation digest of protein expression vector(Day1) |In silico cloning and confirmation digest of protein expression vector]]<br>[[Media:Sp18 M1D1 nll markedV2.pdf| T/R Prelab]]<br>[[Media:Sp18_M1D1_JB_marked.pdf| W/F Prelab]] | | [[20.109(S18):In silico cloning and confirmation digest of protein expression vector(Day1) |In silico cloning and confirmation digest of protein expression vector]]<br>[[Media:Sp18 M1D1 nll markedV2.pdf| T/R Prelab]]<br>[[Media:Sp18_M1D1_JB_marked.pdf| W/F Prelab]] | ||
| <font color=#0174DF>'''Orientation quiz'''</font color> <br> [[20.109(S18):Homework#Due_M1D1| Homework due]] | | <font color=#0174DF>'''Orientation quiz'''</font color> <br> [[20.109(S18):Homework#Due_M1D1| Homework due]] | ||
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| 2 | | 2 | ||
| T/W Feb 13/14 | | T/W Feb 13/14 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:Sp18 20109 M1L2 printout-1.pptx| M1D2 | + | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:Sp18 20109 M1L2 printout-1.pptx| M1D2 Lecture]] |
| [[20.109(S18):Analyze small microarray data and induce protein expression (Day2) |Complete small microarray analysis and induce protein expression]]<br>[[Media:Sp18 M1D2 nll marked.pdf| T/R Prelab]] and [[Media:Sp18 M1D2 nll lig calc.pdf| ligation calculation]]<br>[[Media:Sp18 M1D2 JB marked.pdf| W/F Prelab]] | | [[20.109(S18):Analyze small microarray data and induce protein expression (Day2) |Complete small microarray analysis and induce protein expression]]<br>[[Media:Sp18 M1D2 nll marked.pdf| T/R Prelab]] and [[Media:Sp18 M1D2 nll lig calc.pdf| ligation calculation]]<br>[[Media:Sp18 M1D2 JB marked.pdf| W/F Prelab]] | ||
| [[20.109(S18):Homework#Due_M1D2| Homework due]] | | [[20.109(S18):Homework#Due_M1D2| Homework due]] | ||
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| 3 | | 3 | ||
| R/F Feb 15/16 | | R/F Feb 15/16 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:Sp18 20109 M1L3.pptx| M1D3 | + | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:Sp18 20109 M1L3.pptx| M1D3 Lecture]] |
| [[20.109(S18):Purify protein for secondary assays(Day3) |Purify protein for secondary assays]]<br>[[Media:Sp18 M1D3 nll marked.pdf| T/R Prelab]]<br>[[Media:Sp18 M1D3_JB_marked.pdf| W/F Prelab]] | | [[20.109(S18):Purify protein for secondary assays(Day3) |Purify protein for secondary assays]]<br>[[Media:Sp18 M1D3 nll marked.pdf| T/R Prelab]]<br>[[Media:Sp18 M1D3_JB_marked.pdf| W/F Prelab]] | ||
| [[20.109(S18):Homework#Due_M1D3| Homework due]] | | [[20.109(S18):Homework#Due_M1D3| Homework due]] | ||
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| 4 | | 4 | ||
| R/F Feb 22/23 | | R/F Feb 22/23 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br> [[Media:Sp18 20109 M1L4.pptx| M1D4 Lecture]] |
| [[20.109(S18):Evaluate protein purity and concentration (Day4) |Evaluate protein purity and concentration]]<br>[[Media:Sp18 M1D4_JBmarkedTR.pdf| T/R Prelab]]<br>[[Media:Sp18 M1D3_JBmarkedWF.pdf| W/F Prelab]]<br>[[Media:Sp18_MethodsTips_JB.pdf| Methods Tips]] | | [[20.109(S18):Evaluate protein purity and concentration (Day4) |Evaluate protein purity and concentration]]<br>[[Media:Sp18 M1D4_JBmarkedTR.pdf| T/R Prelab]]<br>[[Media:Sp18 M1D3_JBmarkedWF.pdf| W/F Prelab]]<br>[[Media:Sp18_MethodsTips_JB.pdf| Methods Tips]] | ||
| <font color=#0174DF>'''Laboratory quiz'''</font color> <br> [[20.109(S18):Homework#Due_M1D4| Homework due]] | | <font color=#0174DF>'''Laboratory quiz'''</font color> <br> [[20.109(S18):Homework#Due_M1D4| Homework due]] | ||
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| 5 | | 5 | ||
| T/W Feb 27/28 | | T/W Feb 27/28 | ||
| − | | [http://be.mit.edu/directory/angela-koehler AK]<br> | + | | [http://be.mit.edu/directory/angela-koehler AK]<br>[[Media:Sp18 20109 M1L5.pptx| M1D5 Lecture]] |
| [[20.109(S18):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5) |Test protein activity using peptidyl-prolyl cis-trans isomerase assay]]<br>[[Media:Sp18 M1D5_JBmarkedTR.pdf| T/R Prelab]]<br>[[Media:Sp18 M1D5_JBmarkedWF.pdf| W/F Prelab]] | | [[20.109(S18):Test protein activity using peptidyl-prolyl cis-trans isomerase assay (Day5) |Test protein activity using peptidyl-prolyl cis-trans isomerase assay]]<br>[[Media:Sp18 M1D5_JBmarkedTR.pdf| T/R Prelab]]<br>[[Media:Sp18 M1D5_JBmarkedWF.pdf| W/F Prelab]] | ||
| [[20.109(S18):Homework#Due_M1D5| Homework due]] | | [[20.109(S18):Homework#Due_M1D5| Homework due]] | ||
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| R/F Mar 1/2 | | R/F Mar 1/2 | ||
| [http://be.mit.edu/directory/angela-koehler AK]<br> | | [http://be.mit.edu/directory/angela-koehler AK]<br> | ||
| − | | [[20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6) |Confirm ligand binding using differential scanning fluorimetry assay]]<br> | + | | [[20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6) |Confirm ligand binding using differential scanning fluorimetry assay]]<br>[[Media:Sp18 M1D6 nll.pdf| T/R Prelab |
| [[20.109(S18):Homework#Due_M1D6| Homework due]] | | [[20.109(S18):Homework#Due_M1D6| Homework due]] | ||
|-- | |-- | ||
Revision as of 17:47, 2 March 2018
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (4-149)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)
