Spring 2012:Leanna Morinishi Lab 2
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Contents
Lab 2: Fluorescence Microscopy
Goals
In this module, I am interested in:
- Building and optimizing a fluorescent microscope
- Learning to control the microstepper
- Learning to talk to and control the camera
- Learning more about 3d deconvolution
- Test how it deconvolution is improved with actual PSF vs. theoretical PSF of the microscope
Methodology
Materials
- Pololu A4983 Stepper Motor Driver Carrier with Voltage Regulators[1]
- Thorlabs 8 mm Travel Stepper Motor Actuator[2]
- Matlab
- Matlab Image Acquisition Toolbox[3]
- Matlab Allied Vision Technologies Adaptor[4]
- National Instruments USB Data Acquisition[5]
- Epi-fluorescence Microscope[6] with parts from Thorlabs
- Submicron beads, small enough to be considered point sources
Deconvolution Methods for 3D Fluorescence Microscopy Images[7]
Results
Code
References
- ↑ A4983 Stepper Motor Driver Carrier with Voltage Regulators
- ↑ 8 mm Travel Stepper Motor Actuator
- ↑ Mathworks Image Acquisition Toolbox
- ↑ Matlab Allied Vision Technologies Adaptor
- ↑ National Instruments USB Data Acquisition 6210
- ↑ Lab_Manual:Optical_Microscopy
- ↑ Deconvolution Methods for 3D Fluorescence Microscopy Images - WUSTL 2006