Spring 2012:Leanna Morinishi Lab 2

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20.345: Bioinstrumentation Project Lab

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Lab 2: Fluorescence Microscopy

Goals

In this module, I am interested in:

  • Building and optimizing a fluorescent microscope
  • Learning to control the microstepper
  • Learning to talk to and control the camera
  • Learning more about 3d deconvolution
    • Test how it deconvolution is improved with actual PSF vs. theoretical PSF of the microscope

Methodology

Materials

  • Pololu A4983 Stepper Motor Driver Carrier with Voltage Regulators[1]
  • Thorlabs 8 mm Travel Stepper Motor Actuator[2]
  • Matlab
  • Matlab Image Acquisition Toolbox[3]
  • Matlab Allied Vision Technologies Adaptor[4]
  • National Instruments USB Data Acquisition[5]
  • Epi-fluorescence Microscope[6] with parts from Thorlabs
  • Submicron beads, small enough to be considered point sources

Deconvolution Methods for 3D Fluorescence Microscopy Images[7]

Results

Code

References

  1. ↑ A4983 Stepper Motor Driver Carrier with Voltage Regulators
  2. ↑ 8 mm Travel Stepper Motor Actuator
  3. ↑ Mathworks Image Acquisition Toolbox
  4. ↑ Matlab Allied Vision Technologies Adaptor
  5. ↑ National Instruments USB Data Acquisition 6210
  6. ↑ Lab_Manual:Optical_Microscopy
  7. ↑ Deconvolution Methods for 3D Fluorescence Microscopy Images - WUSTL 2006
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