20.109(F18): Laboratory Fundamentals of Biological Engineering
Module 1
Cell Loading Data
TR and WF cell loading data
CometChip Data
gamma-H2AX Data
Module 2
Fermentation product and gene targeted:
T/R
Team
|
Ethanol (E) or Acetate (A)
|
Gene targeted by CRISPRi gRNA
|
gRNA (DNA) sequence (without tag at 3' end)
|
Locus targeted (eg. beginning of gene, putative promoter, -35 region)
|
Target template or nontemplate strand
|
Colorimetric Assay Results
|
TR red
|
E
|
pta
|
TGCGCCCGATCAGACTACGACTATC
|
Coding region
|
template
|
File:TRRed ethanol rawdata.xlsx
|
TR orange
|
E
|
ldhA
|
gttgcaggtacttcttgtcgt
|
32 bps downstream from start of gene
|
Nontemplate Strand
|
File:TROrange ethanol rawdata.xlsx
|
TR green
|
A
|
adhE
|
TACTAAAAAAGTTTAACATTATCA
|
locus targeted: -34 upstream (promoter)
|
Template strand
|
File:TRGreen acetate rawdata.xlsx
|
TR pink
|
A
|
Citrate Synthase (gltA)
|
tgagttttgcttttgtatcagccat
|
Beginning of gene
|
Non-template Strand
|
File:TRPink acetate rawdata.xlsx
|
TR purple
|
A
|
ldhA
|
TAGTAGCTTAAATGTGATTCAACAT
|
Locus targeted: -40 upstream region (promoter)
|
Non-template strand
|
File:TRPurple acetate rawdata.xlsx
|
W/F
Team
|
Ethanol (E) or Acetate (A)
|
Gene targeted by CRISPRi gRNA
|
gRNA (DNA) sequence (without tag at 3' end)
|
Locus targeted (eg. beginning of gene, putative promoter, -35 region)
|
Target template or nontemplate strand
|
Colorimetric Assay Results
|
WF yellow
|
A
|
adhE
|
ATTCGAGCAGATGATTTACTAAAAA
|
locus targeted: -50 upstream; promoter
|
template strand
|
File:WFyellow Acetate RawData.xlsx
|
WF green
|
Acetate
|
adhE
|
TTACTAAAAAAGTTTAACATTATCA
|
locus targeted: -35 upstream (promoter)
|
template strand
|
File:WFGreen Acetate RawData.xlsx
|
WF blue
|
A
|
adhE
|
TTCGAGCAGATGATTTACTAAA
|
locus targeted: -49 upstream (promoter)
|
Template Strand
|
File:WFBlue Acetate RawData.xlsx
|
gRNA_target Sequencing Results
T/R
W/F
Module 3
Battery Capacity Raw Data
Battery Capacity Raw Data
No-gold battery data
No gold control TEM and EDX images
Experimental battery composition (AuNP/phage)
|
Weight of cathode (mg)
|
Actual capacity (mA*h/g)
|
0
|
2.15 mg
|
95.09
|
0
|
2.37 mg
|
89.47
|
TR Battery information
Team
|
Experimental battery composition (AuNP/phage)
|
Weight of cathode (mg)
|
Actual capacity (mA*h/g)
|
Red
|
|
|
|
Orange
|
|
|
|
Green
|
23
|
3.88
|
85.67201222
|
Pink
|
|
|
|
Purple
|
|
|
|
WF Battery information
Team
|
Experimental battery composition (AuNP/phage)
|
Weight of cathode (mg)
|
Actual capacity (mA*h/g)
|
Yellow
|
|
|
|
Green
|
|
|
|
Blue
|
|
|
|