Difference between revisions of "20.109(F18):Class data"
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Imon Rahaman (Talk | contribs) (→W/F) |
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|'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)''' | |'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)''' | ||
|'''Target template or nontemplate strand''' | |'''Target template or nontemplate strand''' | ||
+ | |'''Colorimetric Assay Results''' | ||
|- | |- | ||
|TR red | |TR red | ||
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| Middle of the operon | | Middle of the operon | ||
| Non-template | | Non-template | ||
+ | |[[File: TRRed_ethanol_rawdata.xlsx | Raw data ]] | ||
|- | |- | ||
|TR orange | |TR orange | ||
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|32 bps downstream from start of gene | |32 bps downstream from start of gene | ||
|Template Strand | |Template Strand | ||
+ | |[[File: TROrange_ethanol_rawdata.xlsx | Raw data ]] | ||
|- | |- | ||
|TR green | |TR green | ||
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|locus targeted: -50 upstream (promoter) | |locus targeted: -50 upstream (promoter) | ||
|Template strand | |Template strand | ||
+ | |[[File: TRGreen_acetate_rawdata.xlsx | Raw data ]] | ||
|- | |- | ||
|TR pink | |TR pink | ||
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|Beginning of gene | |Beginning of gene | ||
|Non-template Strand | |Non-template Strand | ||
+ | |[[File: TRPink_acetate_rawdata.xlsx | Raw data ]] | ||
|- | |- | ||
|TR purple | |TR purple | ||
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|Locus targeted: -40 upstream region (promoter) | |Locus targeted: -40 upstream region (promoter) | ||
|Non-template strand | |Non-template strand | ||
+ | |[[File: TRPurple_acetate_rawdata.xlsx | Raw data ]] | ||
| | | | ||
|} | |} |
Revision as of 16:33, 6 November 2018
Contents
Module 1
Cell Loading Data
CometChip Data
Team | Analyzed Data |
T/R red | Analyzed CometChip data |
T/R orange | Analyzed CometChip data |
T/R green | Analyzed CometChip data |
T/R pink | Analyzed CometChip data |
T/R purple | Analyzed CometChip data |
Team | Analyzed Data |
W/F yellow | Analyzed CometChip data |
W/F green | Analyzed CometChip data |
W/F blue | Analyzed CometChip data |
W/F pink | Analyzed CometChip data |
gamma-H2AX Data
Team | Recovery time (min) | Raw gamma-H2AX Data | Analyzed gamma-H2AX Data |
T/R red | 30 | raw data | analyzed data |
T/R orange | 30 | raw data | analyzed data |
T/R green | 30 | raw data | analyzed data |
T/R pink | 30 | raw data | analyzed data |
T/R purple | 60 | raw data | analyzed data |
Team | Recovery time (min) | Raw gamma-H2AX Data | Analyzed gamma-H2AX Data |
W/F yellow | 30 | raw data | analyzed data |
W/F green | 30 | raw data | analyzed data |
W/F blue | 60 | raw data | analyzed data |
W/F pink | 30 | raw data | analyzed data |
Module 2
Fermentation product and gene targeted:
T/R
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand | Colorimetric Assay Results | |
TR red | E | pta | TGCGCCCGATCAGACTACGACTATC | Middle of the operon | Non-template | File:TRRed ethanol rawdata.xlsx | |
TR orange | E | ldhA | gttgcaggtacttcttgtcgt | 32 bps downstream from start of gene | Template Strand | File:TROrange ethanol rawdata.xlsx | |
TR green | A | adhE | TACTAAAAAAGTTTAACATTATCA | locus targeted: -50 upstream (promoter) | Template strand | File:TRGreen acetate rawdata.xlsx | |
TR pink | A | Citrate Synthase (gltA) | tgagttttgcttttgtatcagccat | Beginning of gene | Non-template Strand | File:TRPink acetate rawdata.xlsx | |
TR purple | A | ldhA | TAGTAGCTTAAATGTGATTCAACAT | Locus targeted: -40 upstream region (promoter) | Non-template strand | File:TRPurple acetate rawdata.xlsx |
W/F
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand |
WF yellow | A | adhE | ATTCGAGCAGATGATTTACTAAAAA | ||
WF green | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA | ||
WF blue | A | adhE | TTCGAGCAGATGATTTACTAAA | locus targeted: -65 upstream (promoter) | Template Strand |
gRNA_target Sequencing Results
T/R
Team | pgRNA sequencing |
red | files |
orange | files |
green | files |
pink | files |
purple | files |
W/F
Team | pgRNA sequencing |
yellow | files |
green | files |
blue | files |