Difference between revisions of "20.109(S24):Spring 2024 schedule"
Becky Meyer (Talk | contribs) |
Noreen Lyell (Talk | contribs) |
||
(71 intermediate revisions by 3 users not shown) | |||
Line 23: | Line 23: | ||
| T/W Feb 6/7 | | T/W Feb 6/7 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp24 Orientation lecture student.pdf| Lecture slides]] | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [[Media:Sp24 Orientation lecture student.pdf| Lecture slides]] | ||
− | | [[20.109(S24):Laboratory tour | Orientation and laboratory tour]]<br> [[Media:Sp24 EHS slides.pdf | EHS slides]]<br> | + | | [[20.109(S24):Laboratory tour | Orientation and laboratory tour]]<br> [[Media:Sp24 EHS slides.pdf | EHS slides]]<br>[[Media:Sp24 M0 nll.pdf| TR prelab slides]] <br> [[Media:Sp24_M0_jz.pdf| WF prelab slides]] |
| | | | ||
|-- | |-- | ||
| M1D1 | | M1D1 | ||
| R/F Feb 8/9 | | R/F Feb 8/9 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] <br> | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L1 2024.pdf| Lecture slides]] |
− | | [[20.109(S24):M1D1 | Complete in-silico cloning of protein expression vector]]<br> | + | | [[20.109(S24):M1D1 | Complete in-silico cloning of protein expression vector]]<br> [[Media:Sp24 M1D1 nll.pdf| TR prelab slides]]<br> [[Media:Sp24 M1D1 jz.pdf| WF prelab slides]] |
| <font color= #3bc2b6 >'''Orientation quiz'''</font color> <br> [[20.109(S24):Homework#Due_M1D1|Homework due]] | | <font color= #3bc2b6 >'''Orientation quiz'''</font color> <br> [[20.109(S24):Homework#Due_M1D1|Homework due]] | ||
|-- | |-- | ||
Line 35: | Line 35: | ||
| T/W Feb 13/14 | | T/W Feb 13/14 | ||
| [http://be.mit.edu/directory/angela-koehler AK] <br> | | [http://be.mit.edu/directory/angela-koehler AK] <br> | ||
− | | [[20.109(S24):M1D2 |Purify expressed protein]] <br> | + | | [[20.109(S24):M1D2 |Purify expressed protein]] <br> [[Media:Sp24 M1D2 nll.pdf| TR prelab slides]] & [https://www.dropbox.com/scl/fi/qq54ep8nhjhwk5xy80x5l/Protein-Purification-Demo.mp4?rlkey=h00htz57i5tt2y9hz8qpieily&dl=0| Protein purification video] <br>[[Media:Sp24 M1D2 jz.pdf| WF prelab slides]] |
| [[20.109(S24):Homework#Due_M1D2|Homework due]] | | [[20.109(S24):Homework#Due_M1D2|Homework due]] | ||
|-- | |-- | ||
| M1D3 | | M1D3 | ||
| R/F Feb 15/16 | | R/F Feb 15/16 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] <br> | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L2 2024.pdf| Lecture slides]] |
− | | [[20.109(S24):M1D3 |Assess purity and concentration of expressed protein]] <br> | + | | [[20.109(S24):M1D3 |Assess purity and concentration of expressed protein]] <br> [[Media:Sp24 M1D3 nll.pdf| TR prelab slides]] <br> [[Media:Sp24 M1D3 jz.pdf| WF prelab slides]] |
| [[20.109(S24):Homework#Due_M1D3|Homework due]] | | [[20.109(S24):Homework#Due_M1D3|Homework due]] | ||
|-- | |-- | ||
Line 52: | Line 52: | ||
| M1D4 | | M1D4 | ||
| R/F Feb 22/23 | | R/F Feb 22/23 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] <br> | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L3 2024.pdf| Lecture slides]] |
− | | [[20.109(S24):M1D4 |Review results of small molecule microarray (SMM) screen]] <br> | + | | [[20.109(S24):M1D4 |Review results of small molecule microarray (SMM) screen]] <br> [[Media:Sp24 M1D4 nll.pdf| TR prelab slides]] <br> [[Media:Sp24_M1D4_jz.pdf| WF prelab slides]] |
| <font color= #3bc2b6 >'''Laboratory quiz'''</font color> <br> [[20.109(S24):Homework#Due_M1D4|Homework due]] | | <font color= #3bc2b6 >'''Laboratory quiz'''</font color> <br> [[20.109(S24):Homework#Due_M1D4|Homework due]] | ||
|-- | |-- | ||
| M1D5 | | M1D5 | ||
| T/W Feb 27/28 | | T/W Feb 27/28 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] <br> | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L4 2024.pdf| Lecture slides]] |
− | | [[20.109(S24):M1D5 | | + | | [[20.109(S24):M1D5 |Setup differential scanning flourimetry (DSF) experiment]] <br> [[Media:Sp24 M1D5v2 nll.pdf| TR prelab slides]]<br>[[Media:Sp24_M1D5_jz.pdf| WF prelab slides]] |
| [[20.109(S24):Homework#Due_M1D5|Homework due]] <br> | | [[20.109(S24):Homework#Due_M1D5|Homework due]] <br> | ||
|-- | |-- | ||
| M1D6 | | M1D6 | ||
| R/F Feb/Mar 29/1 | | R/F Feb/Mar 29/1 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] <br> | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L6 2024 short.pdf| Lecture slides]] |
− | | [[20.109(S24):M1D6 | | + | | [[20.109(S24):M1D6 |Prepare cells for electromobility shift assay (EMSA)]] <br> [[Media:Sp24 M1D6 nll.pdf|TR prelab slides]] <br> [[Media:Sp24 M1D6 jz.pdf|WF prelab slides]] |
− | | [[20.109(S24):Homework#Due_M1D6|Homework due]] <br> [[20.109(S24):Research talk| <font color = #2f9b91>'''Research talk due'''</font color>]] | + | | [[20.109(S24):Homework#Due_M1D6|Homework due]] <br> [[20.109(S24):Research talk| <font color = #2f9b91>'''Research talk due'''</font color>]] Mon, Mar 4 at 10pm <br> |
|-- | |-- | ||
| M1D7 | | M1D7 | ||
| T/W Mar 5/6 | | T/W Mar 5/6 | ||
− | | [http://be.mit.edu/directory/angela-koehler AK] <br> | + | | [http://be.mit.edu/directory/angela-koehler AK] <br> [[Media:20109 M1L7 2024 .pptx| Lecture slides]] |
− | | [[20.109(S24):M1D7 | | + | | [[20.109(S24):M1D7 |Complete EMSA experiment]] <br> [[Media:Sp24 M1D7 nll.pdf| TR prelab slides]]<br> [[Media:Sp24 M1D7 jz.pdf| WF prelab slides]] |
| [[20.109(S24):Homework#Due_M1D7|Homework due]] | | [[20.109(S24):Homework#Due_M1D7|Homework due]] | ||
|-- | |-- | ||
Line 77: | Line 77: | ||
| R/F Mar 7/8 | | R/F Mar 7/8 | ||
| BE Comm Lab <br> | | BE Comm Lab <br> | ||
− | | [[20.109(S24):M1D8 |Evaluate experimental results]] <br> | + | | [[20.109(S24):M1D8 |Evaluate experimental results]] <br> [[Media:Sp24 M1D8v2 nll.pdf| TR & WF prelab slides]] |
| <font color= #3bc2b6 >'''Laboratory quiz'''</font color> <br> [[20.109(S24):Homework#Due_M1D8|Homework due]] | | <font color= #3bc2b6 >'''Laboratory quiz'''</font color> <br> [[20.109(S24):Homework#Due_M1D8|Homework due]] | ||
|-- | |-- | ||
| M2D1 | | M2D1 | ||
| T/W Mar 12/13 | | T/W Mar 12/13 | ||
− | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:Sp24 M2L1.pdf| Lecture slides]] |
− | | [[20.109(S24):M2D1 |Determine peptide design strategy]] <br> | + | | [[20.109(S24):M2D1 |Determine peptide design strategy]] <br> [[Media:Sp24 M2D1 nll.pdf| TR prelab slides]]<br> [[Media:Sp24 M2D1 jz.pdf| WF prelab slides]] |
| [[20.109(S24):Homework#Due_M2D1|Homework due]] | | [[20.109(S24):Homework#Due_M2D1|Homework due]] | ||
|-- | |-- | ||
| M2D2 | | M2D2 | ||
| R/F Mar 14/15 | | R/F Mar 14/15 | ||
− | | [http://be.mit.edu/directory/angela-belcher | + | | [http://be.mit.edu/directory/angela-belcher AMB] <br> [[Media:20170602-GeorgeSun for 20.109 sp 2024.pdf| Lecture slides]] |
− | | [[20.109(S24):M2D2 |Clone cell surface peptide display plasmid]] <br> | + | | [[20.109(S24):M2D2 |Clone cell surface peptide display plasmid]] <br> [[Media:Sp24 M2D2 nll.pdf|TR prelab slides]] <br> [[Media:Sp24 M2D2 nll.pdf|WF prelab slides]] |
| [[20.109(S24):Homework#Due_M2D2|Homework due]] <br> [[20.109(S24):Data Summary| <font color = #2f9b91>'''Data Summary draft due'''</font color>]] Sat, Mar 16 at 10 pm <br> [https://mit.enterprise.slack.com/archives/C06H3BYMX8E Blog post due] Mon, Mar 18 at 10 pm | | [[20.109(S24):Homework#Due_M2D2|Homework due]] <br> [[20.109(S24):Data Summary| <font color = #2f9b91>'''Data Summary draft due'''</font color>]] Sat, Mar 16 at 10 pm <br> [https://mit.enterprise.slack.com/archives/C06H3BYMX8E Blog post due] Mon, Mar 18 at 10 pm | ||
|-- | |-- | ||
| M2D3 | | M2D3 | ||
| T/W Mar 19/20 | | T/W Mar 19/20 | ||
− | | [http://be.mit.edu/directory/angela-belcher | + | | [http://be.mit.edu/directory/angela-belcher AMB] <br> [[Media:Sp24 L2 AMB.pdf | Lecture slides]] |
− | | [[20.109(S24):M2D3 |Sequence clones and transform into yeast]] <br> | + | | [[20.109(S24):M2D3 |Sequence clones and transform into yeast]] <br> [[Media:Sp24 M2D3 nll.pdf|TR prelab slides]]<br> [[Media:Sp24_M2D3_jz.pdf|WF prelab slides]] |
| [[20.109(S24):Homework#Due_M2D3|Homework due]] <br> | | [[20.109(S24):Homework#Due_M2D3|Homework due]] <br> | ||
|-- | |-- | ||
| M2D4 | | M2D4 | ||
| R/F Mar 21/22 | | R/F Mar 21/22 | ||
− | | [http://be.mit.edu/directory/angela-belcher | + | | [http://be.mit.edu/directory/angela-belcher AMB] <br> [[Media:Sp24 L3 AMB.pdf | Lecture slides]] |
− | | [[20.109(S24):M2D4 |Align sequencing and | + | | [[20.109(S24):M2D4 |Align sequencing and prepare for Journal Article presentations]] <br> [[Media:Sp24 TR JADiscussion bcm.pdf |TR prelab slides]] <br> [[Media:UpdateSp24 WF JADiscussion bcm.pdf | WF prelab slides]] |
| <font color= #3bc2b6 >'''Laboratory quiz'''</font color> <br> [[20.109(S24):Homework#Due_M2D4|Homework due]] | | <font color= #3bc2b6 >'''Laboratory quiz'''</font color> <br> [[20.109(S24):Homework#Due_M2D4|Homework due]] | ||
|-- | |-- | ||
Line 124: | Line 124: | ||
| M2D5 | | M2D5 | ||
| T/W Apr 9/10 | | T/W Apr 9/10 | ||
− | | [http://be.mit.edu/directory/angela-belcher | + | | [http://be.mit.edu/directory/angela-belcher AMB] <br> [[Media:20.109 TEM Spring 2024.pdf |Lecture slides]] |
− | | [[20.109(S24):M2D5 | | + | | [[20.109(S24):M2D5 | Perform flow cytometry and harvest cells to test cadmium sequestration]] <br> [[Media: Lecture Slides Flow Cytometry.pdf |TR prelab slides]]<br> [[Media: Sp24 M2D5 jz.pdf |WF prelab slides]] |
| [[20.109(S24):Homework#Due_M2D5|Homework due]] | | [[20.109(S24):Homework#Due_M2D5|Homework due]] | ||
|-- | |-- | ||
| M2D6 | | M2D6 | ||
| R/F Apr 11/12 | | R/F Apr 11/12 | ||
− | | [http://be.mit.edu/directory/angela-belcher | + | | [http://be.mit.edu/directory/angela-belcher AMB] <br> |
− | | [[20.109(S24):M2D6 | Quantify cadmium removal from media]] <br> | + | | [[20.109(S24):M2D6 | Quantify cadmium removal from media]] <br> [[Media:Sp24 M2D6 bcm.pdf | TR prelab slides]] <br> [[Media:Sp24 M2D6 2bcm.pdf | WF prelab slides]] |
| [[20.109(S24):Homework#Due_M2D5|Homework due]] | | [[20.109(S24):Homework#Due_M2D5|Homework due]] | ||
|-- | |-- | ||
Line 143: | Line 143: | ||
| R/F Apr 18/19 | | R/F Apr 18/19 | ||
| [http://be.mit.edu/directory/becky-meyer BCM] <br> | | [http://be.mit.edu/directory/becky-meyer BCM] <br> | ||
− | | [[20.109(S24):M2D7 | Visualize cadmium sequestration and assess quality of cadmium sulfide production]] <br> | + | | [[20.109(S24):M2D7 | Visualize cadmium sequestration and assess quality of cadmium sulfide production]] <br> [[Media:Sp24 M2D7 bcm.pdf | TR prelab slides]]<br> [[Media:Sp24_M2D7_jz.pdf | WF prelab slides]] |
| [[20.109(S24):Homework#Due_M2D7|Homework due]] | | [[20.109(S24):Homework#Due_M2D7|Homework due]] | ||
|-- | |-- | ||
| M2D8 | | M2D8 | ||
| T/W Apr 23/24 | | T/W Apr 23/24 | ||
− | | [http://be.mit.edu/directory/becky-meyer BCM] <br> | + | | [http://be.mit.edu/directory/becky-meyer BCM] <br> [[Media:Sp24 RA discussion bcm.pdf| Lecture slides]] |
− | | [[20.109(S24):M2D8 |Complete data analysis and organize Research Article figures]] <br> | + | | [[20.109(S24):M2D8 |Complete data analysis and organize Research Article figures]] <br> [[Media:Sp24 M2D8 nll.pdf| TR prelab slides]]<br> [[Media:Sp24_M2D8_jz.pdf| WF prelab slides]] |
| <font color= #3bc2b6 >'''Laboratory quiz'''</font color> <br> [[20.109(S24):Homework#Due_M2D8|Homework due]] | | <font color= #3bc2b6 >'''Laboratory quiz'''</font color> <br> [[20.109(S24):Homework#Due_M2D8|Homework due]] | ||
|-- | |-- | ||
| M3D1 | | M3D1 | ||
| R/F Apr 25/26 | | R/F Apr 25/26 | ||
− | | [http://be.mit.edu/directory/angela-belcher | + | | [http://be.mit.edu/directory/angela-belcher AMB] <br> |
− | | [[20.109(S24):M3D1 |Brainstorm ideas for Research proposal presentation]] <br> | + | | [[20.109(S24):M3D1 |Brainstorm ideas for Research proposal presentation]] <br> [[Media:Sp24 M3D1 nll.pdf|TR prelab slides]] <br> [[Media:Sp24 M3D1 nll.pdf|WF prelab slides]] |
| [[20.109(S24):Homework#Due_M3D1|Homework due]] <br> | | [[20.109(S24):Homework#Due_M3D1|Homework due]] <br> | ||
|-- | |-- | ||
Line 161: | Line 161: | ||
| T/W Apr/May 30/1 | | T/W Apr/May 30/1 | ||
| BE Comm Lab | | BE Comm Lab | ||
− | | [[20.109(S24):M3D2 |Pitch research proposal presentation ideas ]] <br> | + | | [[20.109(S24):M3D2 |Pitch research proposal presentation ideas ]] <br> [[Media:Sp24_M3D2_nll.pdf|TR & WF prelab slides]] |
− | | [[20.109(S24):Research article| <font color = #2f9b91>'''Research article due'''</font color>]] | + | | [[20.109(S24):Research article| <font color = #2f9b91>'''Research article due'''</font color>]] Wed, May 1 at 10 pm <br> [https://mit.enterprise.slack.com/archives/C06H3BYMX8E Blog post due] Tue, Apr 30 at 10 pm |
|-- | |-- | ||
| M3D3 | | M3D3 | ||
| R/F May 2/3 | | R/F May 2/3 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL] <br> [http://be.mit.edu/directory/becky-meyer BCM] <br> | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> [http://be.mit.edu/directory/becky-meyer BCM] <br> | ||
− | | [[20.109(S24):M3D3 |Develop ideas for Research proposal presentation ]] <br> | + | | [[20.109(S24):M3D3 |Develop ideas for Research proposal presentation ]] <br> [[Media:Sp24 M3D3 nll.pdf| TR & WF prelab slides]] |
| [[20.109(S24):Homework#Due_M3D3|Homework due]]<br> | | [[20.109(S24):Homework#Due_M3D3|Homework due]]<br> | ||
|-- | |-- | ||
| M3D4 | | M3D4 | ||
| T/W May 7/8 | | T/W May 7/8 | ||
− | | [http://be.mit.edu/directory/ | + | | [http://be.mit.edu/directory/noreen-lyell NLL] <br> |
| [[20.109(S24):M3D4 |Participate in Research proposal peer reviews]] <br> | | [[20.109(S24):M3D4 |Participate in Research proposal peer reviews]] <br> | ||
| [[20.109(S24):Homework#Due_M3D4|Homework due]] | | [[20.109(S24):Homework#Due_M3D4|Homework due]] |
Latest revision as of 18:16, 3 May 2024
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not enough time to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm in 4-237
Laboratory section times: Tuesday (T) and Thursday (R) 1 - 5 pm or Wednesday (W) and Friday (F) 1 - 5 pm in 56-322
ABSENCE POLICY:
Absences from lecture: Attendance will be recorded for participation points throughout the semester. If absent, student is responsible for all information provided in lecture.
Absences from laboratory: Excused absences should be discussed with the Instructors as soon as possible. Because make-up laboratory time is not provided, attendance in another section may be required to complete the necessary experiments. Unexcused absences will result in a 1/3 of a letter grade deduction from the final grade on the major assignment for the module (for example, a B+ would become a B).