Difference between revisions of "20.109(F18):Class data"
From Course Wiki
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|'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)''' | |'''Locus targeted (eg. beginning of gene, putative promoter, -35 region)''' | ||
|'''Target template or nontemplate strand''' | |'''Target template or nontemplate strand''' | ||
+ | |'''Colorimetric Assay Results''' | ||
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|WF yellow | |WF yellow | ||
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+ | |[[File: WFYellow_acetate_rawdata.xlsx | Raw data ]] | ||
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|WF green | |WF green | ||
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+ | |[[File: WFGreen_acetate_rawdata.xlsx | Raw data ]] | ||
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|WF blue | |WF blue | ||
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| locus targeted: -65 upstream (promoter) | | locus targeted: -65 upstream (promoter) | ||
| Template Strand | | Template Strand | ||
+ | |[[File: WFBlue_acetate_rawdata.xlsx | Raw data ]] | ||
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Revision as of 16:34, 6 November 2018
Contents
Module 1
Cell Loading Data
CometChip Data
Team | Analyzed Data |
T/R red | Analyzed CometChip data |
T/R orange | Analyzed CometChip data |
T/R green | Analyzed CometChip data |
T/R pink | Analyzed CometChip data |
T/R purple | Analyzed CometChip data |
Team | Analyzed Data |
W/F yellow | Analyzed CometChip data |
W/F green | Analyzed CometChip data |
W/F blue | Analyzed CometChip data |
W/F pink | Analyzed CometChip data |
gamma-H2AX Data
Team | Recovery time (min) | Raw gamma-H2AX Data | Analyzed gamma-H2AX Data |
T/R red | 30 | raw data | analyzed data |
T/R orange | 30 | raw data | analyzed data |
T/R green | 30 | raw data | analyzed data |
T/R pink | 30 | raw data | analyzed data |
T/R purple | 60 | raw data | analyzed data |
Team | Recovery time (min) | Raw gamma-H2AX Data | Analyzed gamma-H2AX Data |
W/F yellow | 30 | raw data | analyzed data |
W/F green | 30 | raw data | analyzed data |
W/F blue | 60 | raw data | analyzed data |
W/F pink | 30 | raw data | analyzed data |
Module 2
Fermentation product and gene targeted:
T/R
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand | Colorimetric Assay Results | |
TR red | E | pta | TGCGCCCGATCAGACTACGACTATC | Middle of the operon | Non-template | File:TRRed ethanol rawdata.xlsx | |
TR orange | E | ldhA | gttgcaggtacttcttgtcgt | 32 bps downstream from start of gene | Template Strand | File:TROrange ethanol rawdata.xlsx | |
TR green | A | adhE | TACTAAAAAAGTTTAACATTATCA | locus targeted: -50 upstream (promoter) | Template strand | File:TRGreen acetate rawdata.xlsx | |
TR pink | A | Citrate Synthase (gltA) | tgagttttgcttttgtatcagccat | Beginning of gene | Non-template Strand | File:TRPink acetate rawdata.xlsx | |
TR purple | A | ldhA | TAGTAGCTTAAATGTGATTCAACAT | Locus targeted: -40 upstream region (promoter) | Non-template strand | File:TRPurple acetate rawdata.xlsx |
W/F
Team | Ethanol (E) or Acetate (A) | Gene targeted by CRISPRi gRNA | gRNA (DNA) sequence (without tag at 3' end) | Locus targeted (eg. beginning of gene, putative promoter, -35 region) | Target template or nontemplate strand | Colorimetric Assay Results |
WF yellow | A | adhE | ATTCGAGCAGATGATTTACTAAAAA | Raw data | ||
WF green | Acetate | adhE | TTACTAAAAAAGTTTAACATTATCA | Raw data | ||
WF blue | A | adhE | TTCGAGCAGATGATTTACTAAA | locus targeted: -65 upstream (promoter) | Template Strand | Raw data |
gRNA_target Sequencing Results
T/R
Team | pgRNA sequencing |
red | files |
orange | files |
green | files |
pink | files |
purple | files |
W/F
Team | pgRNA sequencing |
yellow | files |
green | files |
blue | files |