Talk:20.109(F16):Module 1

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20.109(F16): Laboratory Fundamentals of Biological Engineering

Engelward PNAS 2006.png

Schedule Fall 2016        Announcements        Assignments        Homework        Communication
       1. Measuring Genomic Instability        2. Manipulating Metabolism        3. Engineering Biomaterials              

Module 1 Data

M1D2: cell loading parameters

All teams' choices are recorded here.
The images from the doubling time experiments, CometChip t = 0 (see below), are illustrative of how many cells were loaded per microwell under each condition.

M1D2: doubling time, pictures and fluorescence signal

The 'data' below refer to Excel spreadsheets

  • listing the fluorescence signal (a.u., with 1 cell corresponding to ~ 1600 a.u.) detected in each microwell in the field of view (rows 8 and above),
  • together with the average signal in representative macrowells of quadrants A1, A2, B1, and B2 (row 5; see loading conditions above),
  • and with the corresponding calculated doubling times (row 6).

One image per quadrant was recorded. Open images in ImageJ for all encoded information to be displayed.

team data summary t=0 and t=2.5d images
T/R yellow data images
T/R green data images
T/R blue data images
T/R pink data images
T/R purple data images
W/F red data images
W/F green data images
W/F blue data images
W/F purple data images

M1D3: H2O2 and MMS dose response pictures and comet analysis

One image per macrowell was collected. Open images in ImageJ for all encoded information to be displayed.

The 'data' below refer to Excel spreadsheets in which

  • rows A, B, C were triplicates in 'live' CometChips,
  • rows D, E, F in 'dead' CometChips,
  • and columns 1-6 correspond to the 6 drug doses the cells were treated with:
    • H2O2: 0, 10, 20, 40, 60, and 80 μM
    • MMS: 0, 0.25, 0.5, 1, 2, and 4 mM.
  • The 'comet number' tab indicates how many comets were analyzed in each image.
  • The other tabs represent various options to quantify DNA damage.
team data summary images
T/R yellow data images
T/R green data images
T/R blue data images
T/R pink data images
T/R purple data images
W/F red data images
W/F green data images
W/F blue data images
W/F purple data images

M1D4: DNA repair kinetics of Coriell cells, pictures and comet analysis

One image per macrowell was collected. Open images in ImageJ for all encoded information to be displayed.

After analyzing your team's data on M1D5,

  • use the 'Upload file' link on the far left to load your final Excel spreadsheet onto the wiki, and
  • link it to the below table.
team data summary images
T/R yellow data images
T/R green data images
T/R blue data images
T/R pink data images
T/R purple data images
W/F red data images
W/F green data images
W/F blue data images
W/F purple data images
TK6 your turn! images