Difference between revisions of "Talk:20.109(F17):Module 2"

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===M2D5/M2D7: sequencing data===
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====T/R====
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{| border=1px
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|'''Team'''
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|'''pgRNA sequencing'''
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|-
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|yellow
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|[[Media:TR Yellow.zip| files]]
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|-
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|green
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|[[Media:TR Green.zip| files]]
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|-
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|blue
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|[[Media:TR Blue.zip| files]]
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|-
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|pink
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|[[Media:TR Pink.zip| files]]
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|-
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|purple
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|[[Media:TR Purple.zip| files]]
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|}
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====W/F====
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{| border=1px
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|'''Team'''
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|'''pgRNA sequencing'''
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|-
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|red
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|[[Media:WFred_sequencing.zip| files]]
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|-
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|green
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|[[Media:WFgreen_sequencing.zip| files]]
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|-
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|blue
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|[[Media:WFblue_sequencing.zip| files]]
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|-
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|purple
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|[[Media:WFpurple_sequencing.zip| files]]
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|-
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|instructors
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|[[Media:Instructors_ackB.zip| ackB files]]
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|-
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|instructors
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|[[Media:Instructors_ldhA.zip| ldhA files]]
 
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Revision as of 14:41, 2 November 2017

Fermentation product and gene targeted:

T/R

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target template or nontemplate strand
red Acetate adhE TTAACGCACTCGTAGAGCGTGTAAA
orange Ethanol pta-ack CTATGGCTCCCTGACGTTTT
yellow Ethanol frdA GCTGTGGGATAAAAACAATCTGGAG
green E LdhA ttgtgctataaacggcgagtttcat
blue E pta ttcacgacaacgttcaataatcat
pink Acetate adhE TTACTAAAAAAGTTTAACATTATCA
purple Acetate adhE TTTACTAAAAAAGTTTAACATTATC
white Acetate adhE CTGATAATGTTAAACTTTTT

W/F

Team Ethanol (E) or Acetate (A) Gene targeted by CRISPRi gRNA gRNA sequence (without tag at 3' end) Locus targeted (eg. beginning of gene, putative promoter, -35 region) Target template or nontemplate strand
red Ethanol ack (indirectly, pta) GTTTTTTTAGCCACGTATCAATTAT
orange Ethanol ldhA ATTCAACATCACTGGAGAAAGTCTT
blue Ethanol ackA TTTTTAGCCACGTATCAATTAT

M2D5/M2D7: sequencing data

T/R

Team pgRNA sequencing
yellow files
green files
blue files
pink files
purple files

W/F

Team pgRNA sequencing
red files
green files
blue files
purple files
instructors ackB files
instructors ldhA files
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