Difference between revisions of "Talk:20.109(F16):Module 2"
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|ldhA | |ldhA | ||
|GTAGCTTAAATGTGATTCAACATC | |GTAGCTTAAATGTGATTCAACATC | ||
− | | | + | |putative promoter |
|- | |- | ||
|blue | |blue | ||
|fdrA | |fdrA | ||
|CAAGATCGGCTTGAAAGGTTTGCAC | |CAAGATCGGCTTGAAAGGTTTGCAC | ||
− | | | + | |Beginning of gene |
|- | |- | ||
|purple | |purple | ||
|ldhA | |ldhA | ||
|TCGTACTGTTTTGTGCTATAAA | |TCGTACTGTTTTGTGCTATAAA | ||
− | | | + | |Beginning of coding sequence |
|- | |- | ||
− | |instructors | + | |instructors<br><font color='red'>red</font color><br><font color='blue'>blue</font color> |
|ackB | |ackB | ||
|AGTACCTATAATTGATACGTGGCTA | |AGTACCTATAATTGATACGTGGCTA | ||
Line 121: | Line 121: | ||
|purple | |purple | ||
|[[Media:WFpurple_sequencing.zip| files]] | |[[Media:WFpurple_sequencing.zip| files]] | ||
+ | |- | ||
+ | |instructors | ||
+ | |[[Media:Instructors_ackB.zip| ackB files]] | ||
+ | |- | ||
+ | |instructors | ||
+ | |[[Media:Instructors_ldhA.zip| ldhA files]] | ||
|} | |} | ||
+ | |||
+ | ===M2D8: mixed-acid fermentation data=== | ||
+ | ====T/R: lactate==== | ||
+ | [[Media: Fa16_TR_Mod2_data_compiled.xlsx| TR lactate data.xlsx]] | ||
+ | ====W/F: ethanol==== | ||
+ | [[Media: WF ethanol data 161109.xlsx| WF ethanol data.xlsx]] | ||
+ | |||
+ | [[Media: WF ethanol data 161109-3.xlsx| WF ethanol data.xlsx - Update to Green data]] |
Latest revision as of 17:34, 15 November 2016
Contents
Module 2 Data
M2D2: gRNA design
T/R: lactate
Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
yellow | pflB | TGTCGAAGTACGCAGTAAAT | putative promoter |
green | pflB | ATAAAAAATCCACTTAAGAAGGTA | Putative Promoter |
blue | pflB | TTCATTAAGCTCGGACATGTAACA | End of Putative Promoter & beginning of pflB gene |
pink | pflB | AAATAAAAAATCCACTTAAGAAGGT | Putative Promoter |
purple | pflB | AAATCCACTTAAGAAGGTAGGTGT | putative promoter |
W/F: ethanol
Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
red | pta-ack | GCC ACG TAT CAA TTA TAG GTA C | putative promoter |
green | ldhA | GTAGCTTAAATGTGATTCAACATC | putative promoter |
blue | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC | Beginning of gene |
purple | ldhA | TCGTACTGTTTTGTGCTATAAA | Beginning of coding sequence |
instructors red blue |
ackB | AGTACCTATAATTGATACGTGGCTA | promoter, -30 region |
instructors | ldhA | CTTAAATGTGATTCAACATCACTG | 5'UTR |
Note: The dCas9 recognition sequence is 5' - GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC - 3'.
M2D5/M2D7: sequencing data
T/R
Team | pgRNA sequencing |
yellow | files |
green | files |
blue | files |
pink | files |
purple | files |
W/F
Team | pgRNA sequencing |
red | files |
green | files |
blue | files |
purple | files |
instructors | ackB files |
instructors | ldhA files |