Talk:20.109(F16):Module 2
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Module 2 Data
M2D2: gRNA design
T/R: lactate
| Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| yellow | pflB | TGTCGAAGTACGCAGTAAAT | putative promoter |
| green | pflB | ATAAAAAATCCACTTAAGAAGGTA | Putative Promoter |
| blue | pflB | TTCATTAAGCTCGGACATGTAACA | End of Putative Promoter & beginning of pflB gene |
| pink | pflB | AAATAAAAAATCCACTTAAGAAGGT | Putative Promoter |
| purple | pflB | AAATCCACTTAAGAAGGTAGGTGT | putative promoter |
W/F: ethanol
| Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| red | pta-ack | GCC ACG TAT CAA TTA TAG GTA C | putative promoter |
| green | ldhA | GTAGCTTAAATGTGATTCAACATC | putative promoter |
| blue | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC | Beginning of gene |
| purple | ldhA | TCGTACTGTTTTGTGCTATAAA | Beginning of coding sequence |
| instructors red blue |
ackB | AGTACCTATAATTGATACGTGGCTA | promoter, -30 region |
| instructors | ldhA | CTTAAATGTGATTCAACATCACTG | 5'UTR |
Note: The dCas9 recognition sequence is 5' - GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC - 3'.
M2D5/M2D7: sequencing data
T/R
| Team | pgRNA sequencing |
| yellow | files |
| green | files |
| blue | files |
| pink | files |
| purple | files |
W/F
| Team | pgRNA sequencing |
| red | files |
| green | files |
| blue | files |
| purple | files |
| instructors | ackB files |
| instructors | ldhA files |
