Difference between revisions of "Talk:20.109(F16):Module 2"
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| Line 21: | Line 21: | ||
|pflB | |pflB | ||
|ATAAAAAATCCACTTAAGAAGGTA | |ATAAAAAATCCACTTAAGAAGGTA | ||
| − | | | + | |Putative Promoter |
|- | |- | ||
|blue | |blue | ||
| Line 36: | Line 36: | ||
|pflB | |pflB | ||
|AAATCCACTTAAGAAGGTAGGTGT | |AAATCCACTTAAGAAGGTAGGTGT | ||
| − | | | + | |putative promoter |
|} | |} | ||
| Line 50: | Line 50: | ||
|pta-ack | |pta-ack | ||
|GCC ACG TAT CAA TTA TAG GTA C | |GCC ACG TAT CAA TTA TAG GTA C | ||
| − | | | + | |putative promoter |
|- | |- | ||
|green | |green | ||
|ldhA | |ldhA | ||
|GTAGCTTAAATGTGATTCAACATC | |GTAGCTTAAATGTGATTCAACATC | ||
| − | | | + | |putative promoter |
|- | |- | ||
|blue | |blue | ||
|fdrA | |fdrA | ||
|CAAGATCGGCTTGAAAGGTTTGCAC | |CAAGATCGGCTTGAAAGGTTTGCAC | ||
| − | | | + | |Beginning of gene |
|- | |- | ||
|purple | |purple | ||
|ldhA | |ldhA | ||
|TCGTACTGTTTTGTGCTATAAA | |TCGTACTGTTTTGTGCTATAAA | ||
| − | | | + | |Beginning of coding sequence |
| + | |- | ||
| + | |instructors<br><font color='red'>red</font color><br><font color='blue'>blue</font color> | ||
| + | |ackB | ||
| + | |AGTACCTATAATTGATACGTGGCTA | ||
| + | |promoter, -30 region | ||
| + | |- | ||
| + | |instructors | ||
| + | |ldhA | ||
| + | |CTTAAATGTGATTCAACATCACTG | ||
| + | |5'UTR | ||
|} | |} | ||
| Line 111: | Line 121: | ||
|purple | |purple | ||
|[[Media:WFpurple_sequencing.zip| files]] | |[[Media:WFpurple_sequencing.zip| files]] | ||
| + | |- | ||
| + | |instructors | ||
| + | |[[Media:Instructors_ackB.zip| ackB files]] | ||
| + | |- | ||
| + | |instructors | ||
| + | |[[Media:Instructors_ldhA.zip| ldhA files]] | ||
|} | |} | ||
| + | |||
| + | ===M2D8: mixed-acid fermentation data=== | ||
| + | ====T/R: lactate==== | ||
| + | [[Media: Fa16_TR_Mod2_data_compiled.xlsx| TR lactate data.xlsx]] | ||
| + | ====W/F: ethanol==== | ||
| + | [[Media: WF ethanol data 161109.xlsx| WF ethanol data.xlsx]] | ||
| + | |||
| + | [[Media: WF ethanol data 161109-3.xlsx| WF ethanol data.xlsx - Update to Green data]] | ||
Latest revision as of 17:34, 15 November 2016
Contents
Module 2 Data
M2D2: gRNA design
T/R: lactate
| Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| yellow | pflB | TGTCGAAGTACGCAGTAAAT | putative promoter |
| green | pflB | ATAAAAAATCCACTTAAGAAGGTA | Putative Promoter |
| blue | pflB | TTCATTAAGCTCGGACATGTAACA | End of Putative Promoter & beginning of pflB gene |
| pink | pflB | AAATAAAAAATCCACTTAAGAAGGT | Putative Promoter |
| purple | pflB | AAATCCACTTAAGAAGGTAGGTGT | putative promoter |
W/F: ethanol
| Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| red | pta-ack | GCC ACG TAT CAA TTA TAG GTA C | putative promoter |
| green | ldhA | GTAGCTTAAATGTGATTCAACATC | putative promoter |
| blue | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC | Beginning of gene |
| purple | ldhA | TCGTACTGTTTTGTGCTATAAA | Beginning of coding sequence |
| instructors red blue |
ackB | AGTACCTATAATTGATACGTGGCTA | promoter, -30 region |
| instructors | ldhA | CTTAAATGTGATTCAACATCACTG | 5'UTR |
Note: The dCas9 recognition sequence is 5' - GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGC - 3'.
M2D5/M2D7: sequencing data
T/R
| Team | pgRNA sequencing |
| yellow | files |
| green | files |
| blue | files |
| pink | files |
| purple | files |
W/F
| Team | pgRNA sequencing |
| red | files |
| green | files |
| blue | files |
| purple | files |
| instructors | ackB files |
| instructors | ldhA files |
