Revision as of 14:19, 2 November 2016

20.109(F16): Laboratory Fundamentals of Biological Engineering

Schedule Fall 2016 Announcements Assignments Homework Communication
1. Measuring Genomic Instability 2. Manipulating Metabolism 3. Engineering Biomaterials

Module 2 Data

Team	Gene targeted by CRISPRi gRNA	gRNA sequence (without tag at 3' end)	Locus targeted (e.g. beginning of gene, putative promoter, -35 region)
yellow	pflB	TGTCGAAGTACGCAGTAAAT
green	pflB	ATAAAAAATCCACTTAAGAAGGTA
blue	pflB	TTCATTAAGCTCGGACATGTAACA
pink	pflB	AAATAAAAAATCCACTTAAGAAGGT
purple	pflB	AAATCCACTTAAGAAGGTAGGTGT

Team	Gene targeted by CRISPRi gRNA	gRNA sequence (without tag at 3' end)	Locus targeted (e.g. beginning of gene, putative promoter, -35 region)
red	pta-ack	GCC ACG TAT CAA TTA TAG GTA C
green	ldhA	GTAGCTTAAATGTGATTCAACATC
blue	fdrA	CAAGATCGGCTTGAAAGGTTTGCAC
purple	ldhA	TCGTACTGTTTTGTGCTATAAA

@@ Line 4: / Line 4: @@
 =<center>Module 2 Data</center>=
 ===M2D2: gRNA design===
-===Fermentation product and gene targeted:===
 ====T/R: lactate====
@@ Line 11: / Line 9: @@
 {| border=1px
 |'''Team'''
-|'''Gene targeted <br> by CRISPRi gRNA'''
+|'''Gene targeted by <br> CRISPRi gRNA'''
 |'''gRNA sequence <br> (without tag at 3' end)'''
 |'''Locus targeted <br> (''e.g.'' beginning of gene, <br>putative promoter, -35 region)
@@ Line 45: / Line 43: @@
 {| border=1px
 |'''Team'''
-|'''Gene targeted by CRISPRi gRNA'''
+|'''Gene targeted by <br>CRISPRi gRNA'''
-|'''gRNA sequence (without tag at 3' end)'''
+|'''gRNA sequence <br>(without tag at 3' end)'''
-|'''Locus targeted <br>(''e.g.'' beginning of gene, putative promoter, -35 region)
+|'''Locus targeted <br>(''e.g.'' beginning of gene, <br>putative promoter, -35 region)
 |-
 |red