Difference between revisions of "Talk:20.109(F16):Module 2"
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{| border=1px | {| border=1px | ||
|'''Team''' | |'''Team''' | ||
| − | |'''Gene targeted by CRISPRi gRNA''' | + | |'''Gene targeted <br> by CRISPRi gRNA''' |
| − | |'''gRNA sequence (without tag at 3' end)''' | + | |'''gRNA sequence <br> (without tag at 3' end)''' |
| − | |'''Locus targeted <br>(''e.g.'' beginning of gene, putative promoter, -35 region) | + | |'''Locus targeted <br> (''e.g.'' beginning of gene, <br>putative promoter, -35 region) |
|- | |- | ||
|yellow | |yellow | ||
Revision as of 14:18, 2 November 2016
Contents
Module 2 Data
M2D2: gRNA design
Fermentation product and gene targeted:
T/R: lactate
| Team | Gene targeted by CRISPRi gRNA |
gRNA sequence (without tag at 3' end) |
Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| yellow | pflB | TGTCGAAGTACGCAGTAAAT | |
| green | pflB | ATAAAAAATCCACTTAAGAAGGTA | |
| blue | pflB | TTCATTAAGCTCGGACATGTAACA | |
| pink | pflB | AAATAAAAAATCCACTTAAGAAGGT | |
| purple | pflB | AAATCCACTTAAGAAGGTAGGTGT |
W/F: ethanol
| Team | Gene targeted by CRISPRi gRNA | gRNA sequence (without tag at 3' end) | Locus targeted (e.g. beginning of gene, putative promoter, -35 region) |
| red | pta-ack | GCC ACG TAT CAA TTA TAG GTA C | |
| green | ldhA | GTAGCTTAAATGTGATTCAACATC | |
| blue | fdrA | CAAGATCGGCTTGAAAGGTTTGCAC | |
| purple | ldhA | TCGTACTGTTTTGTGCTATAAA |
