Talk:20.109(F16):Module 1

M1D2: cell loading parameters

All teams' choices are recorded here.
The images from the doubling time experiments, CometChip t = 0 (see below), are illustrative of how many cells were loaded per microwell under each condition.

M1D2: doubling time, pictures and fluorescence signal

The 'data' below refer to Excel spreadsheets

• listing the fluorescence signal (a.u., with 1 cell corresponding to ~ 1600 a.u.) detected in each microwell in the field of view (rows 8 and above),
• together with the average signal in representative macrowells of quadrants A1, A2, B1, and B2 (row 5; see loading conditions above),
• and with the corresponding calculated doubling times (row 6).

One image per quadrant was recorded.

team	data summary	t=0 and t=2.5 images
T/R yellow	data	images
T/R green	data	images
T/R blue	data	images
T/R pink	data	images
T/R purple	data	images
W/F red	data	images
W/F green	data	images
W/F blue	data	images
W/F purple	data	images

M1D3: H2O2 and MMS dose response pictures and comet analysis

The 'data' below refer to Excel spreadsheets in which

• rows A, B, C were triplicates in 'live' CometChips,
• rows D, E, F in 'dead' CometChips,
• and columns 1-6 correspond to the 6 drug doses the cells were treated with:
  • H₂O₂: 0, 10, 20, 40, 60, and 80 μM
  • MMS: 0, 0.25, 0.5, 1, 2, and 4 mM.

One image per macrowell was collected.

• The 'comet number' tab indicates how many comets were analyzed in each image.
• The other tabs represent various options to quantify DNA damage.

team	data summary	images
T/R yellow	data	images
T/R green	data	images
T/R blue	data	images
T/R pink	data	images
T/R purple	data	images
W/F red	data	images
W/F green	data	images
W/F blue	data	images
W/F purple	data	images