Difference between revisions of "Talk:20.109(F16):Module 1"

M1D2: cell loading parameters

All teams' choices are recorded here.
The images from the doubling time experiments, CometChip t = 0 (see below), are illustrative of how many cells were loaded per microwell under each condition.

M1D2: doubling time, pictures and fluorescence signal

The 'data' below refer to Excel spreadsheets

• listing the fluorescence signal (a.u., with 1 cell corresponding to ~ 1600 a.u.) detected in each microwell in the field of view (rows 8 and above),
• together with the average signal in representative macrowells of quadrants A1, A2, B1, and B2 (row 5; see loading conditions above),
• and with the corresponding calculated doubling times (row 6).

One image per quadrant was recorded. Open the images in ImageJ for all the encoded information to be displayed.

M1D3: H2O2 and MMS dose response pictures and comet analysis

One image per macrowell was collected. Open the images in ImageJ for all the encoded information to be displayed.
The 'data' below refer to Excel spreadsheets in which

• rows A, B, C were triplicates in 'live' CometChips,
• rows D, E, F in 'dead' CometChips,
• and columns 1-6 correspond to the 6 drug doses the cells were treated with:
  • H₂O₂: 0, 10, 20, 40, 60, and 80 μM
  • MMS: 0, 0.25, 0.5, 1, 2, and 4 mM.
• The 'comet number' tab indicates how many comets were analyzed in each image.
• The other tabs represent various options to quantify DNA damage.