Difference between revisions of "Talk:20.109(F16):Module 1"
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MAXINE JONAS (Talk | contribs) (→M1D2: doubling time, pictures and fluorescence signal) |
MAXINE JONAS (Talk | contribs) (→M1D3: H2O2 and MMS dose response pictures and comet analysis) |
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**H<sub>2</sub>O<sub>2</sub>: 0, 10, 20, 40, 60, and 80 μM | **H<sub>2</sub>O<sub>2</sub>: 0, 10, 20, 40, 60, and 80 μM | ||
**MMS: 0, 0.25, 0.5, 1, 2, and 4 mM. | **MMS: 0, 0.25, 0.5, 1, 2, and 4 mM. | ||
− | One image per macrowell was collected. | + | One image per macrowell was collected. '''Open the images in ImageJ''' for all the encoded information to be displayed. |
*The 'comet number' tab indicates how many comets were analyzed in each image. | *The 'comet number' tab indicates how many comets were analyzed in each image. | ||
*The other tabs represent various options to quantify DNA damage. | *The other tabs represent various options to quantify DNA damage. |
Revision as of 15:54, 23 September 2016
M1D2: cell loading parameters
All teams' choices are recorded here.
The images from the doubling time experiments, CometChip t = 0 (see below), are illustrative of how many cells were loaded per microwell under each condition.
M1D2: doubling time, pictures and fluorescence signal
The 'data' below refer to Excel spreadsheets
- listing the fluorescence signal (a.u., with 1 cell corresponding to ~ 1600 a.u.) detected in each microwell in the field of view (rows 8 and above),
- together with the average signal in representative macrowells of quadrants A1, A2, B1, and B2 (row 5; see loading conditions above),
- and with the corresponding calculated doubling times (row 6).
One image per quadrant was recorded. Open the images in ImageJ for all the encoded information to be displayed.
team | data summary | t=0 and t=2.5 images |
T/R yellow | data | images |
T/R green | data | images |
T/R blue | data | images |
T/R pink | data | images |
T/R purple | data | images |
W/F red | data | images |
W/F green | data | images |
W/F blue | data | images |
W/F purple | data | images |
M1D3: H2O2 and MMS dose response pictures and comet analysis
The 'data' below refer to Excel spreadsheets in which
- rows A, B, C were triplicates in 'live' CometChips,
- rows D, E, F in 'dead' CometChips,
- and columns 1-6 correspond to the 6 drug doses the cells were treated with:
- H2O2: 0, 10, 20, 40, 60, and 80 μM
- MMS: 0, 0.25, 0.5, 1, 2, and 4 mM.
One image per macrowell was collected. Open the images in ImageJ for all the encoded information to be displayed.
- The 'comet number' tab indicates how many comets were analyzed in each image.
- The other tabs represent various options to quantify DNA damage.
team | data summary | images |
T/R yellow | data | images |
T/R green | data | images |
T/R blue | data | images |
T/R pink | data | images |
T/R purple | data | images |
W/F red | data | images |
W/F green | data | images |
W/F blue | data | images |
W/F purple | data | images |