Difference between revisions of "Talk:20.109(F16):Module 1"

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(→‎M1D2: doubling time, pictures and fluorescence signal)
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===M1D3: H2O2 and MMS dose response pictures and comet analysis===
 
===M1D3: H2O2 and MMS dose response pictures and comet analysis===
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{| border=1px
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|'''team'''
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|'''data summary'''
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|'''images'''
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|-
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|T/R yellow
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|[[Media:160913TK6_H2O2_6Doses_TRyellow.xlsx| data]]
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|[[Media:TRyellow.zip| images]]
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|-
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|T/R green
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|[[Media:160913TK6_H2O2_6Doses_TRgreen.xlsx| data]]
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|[[Media:TRgreen.zip| images]]
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|-
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|T/R blue
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|[[Media:160913TK6_H2O2_6Doses_TRblue.xlsx| data]]
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|[[Media:TRblue.zip| images]]
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|-
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|T/R pink
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|[[Media:160913TK6_H2O2_6Doses_TRpink.xlsx| data]]
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|[[Media:TRpink.zip| images]]
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|-
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|T/R purple
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|[[Media:160913TK6_H2O2_6Doses_TRpurple.xlsx| data]]
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|[[Media:TRpurple.zip| images]]
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|-
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|W/F red
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|[[Media:160914TK6_MMS_6Doses_WFred.xlsx| data]]
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|[[Media:WFred.zip| images]]
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|-
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|W/F green
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|[[Media:160914TK6_MMS_6Doses_WFgreen.xlsx| data]]
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|[[Media:WFgreen.zip| images]]
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|-
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|W/F blue
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|[[Media:160914TK6_MMS_6Doses_WFblue.xlsx| data]]
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|[[Media:WFblue.zip| images]]
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|-
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|W/F purple
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|[[Media:160914TK6_MMS_6Doses_WFpurple.xlsx| data]]
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|[[Media:WFpurple.zip| images]]
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|-
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|}

Revision as of 15:31, 23 September 2016

20.109(F16): Laboratory Fundamentals of Biological Engineering

Engelward PNAS 2006.png

Schedule Fall 2016        Announcements        Assignments        Homework        Communication
       1. Measuring Genomic Instability        2. Manipulating Metabolism        3. Engineering Biomaterials              

M1D2: cell loading parameters

All teams' choices are recorded here.
The images from the doubling time experiments, CometChip t = 0 (see below), are illustrative of how many cells were loaded per microwell under each condition.

M1D2: doubling time, pictures and fluorescence signal

The data below refers to Excel spreadsheets

  • listing the fluorescence signal (a.u., with 1 cell corresponding to ~ 1600 a.u.) detected in each microwell in the field of view (rows 8 and above),
  • together with the average signal in representative macrowells of quadrants A1, A2, B1, and B2 (row 5; see loading conditions above),
  • and with the corresponding calculated doubling times (row 6).

One image per quadrant was recorded.

team data summary t=0 and t=2.5 images
T/R yellow data images
T/R green data images
T/R blue data images
T/R pink data images
T/R purple data images
W/F red data images
W/F green data images
W/F blue data images
W/F purple data images

M1D3: H2O2 and MMS dose response pictures and comet analysis

team data summary images
T/R yellow data images
T/R green data images
T/R blue data images
T/R pink data images
T/R purple data images
W/F red data images
W/F green data images
W/F blue data images
W/F purple data images
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