Difference between revisions of "20.109(S20): Spring 2020 schedule"

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| R/F Apr 14/15  
 
| R/F Apr 14/15  
| [http://be.mit.edu/directory/leslie-mcclain LM]<br>
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| Comm Lab<br>
 
| [[20.109(S20):Growth of phage-based active (cathode) material (Day1) | Grow phage-based active (cathode) material]]<br>
 
| [[20.109(S20):Growth of phage-based active (cathode) material (Day1) | Grow phage-based active (cathode) material]]<br>
 
| [[20.109(S20):Homework#Due_M3D1| Homework due]] <br> [[20.109(S20): Research article| <font color=#B40404>'''Research article due'''</font color>]] Sat, Apr 20 at 10 pm <br> [https:// Blog post due] Sun, Apr 21 at 10 pm
 
| [[20.109(S20):Homework#Due_M3D1| Homework due]] <br> [[20.109(S20): Research article| <font color=#B40404>'''Research article due'''</font color>]] Sat, Apr 20 at 10 pm <br> [https:// Blog post due] Sun, Apr 21 at 10 pm
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| T/W Apr 23/24
| [http://be.mit.edu/directory/angela-belcher AB]<br>
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| [http://be.mit.edu/directory/leslie-mcclain LM]<br>
 
| [[20.109(S20):Construct cathode with phage-based active material (Day3)| Construct cathode with phage-based active material]]<br>
 
| [[20.109(S20):Construct cathode with phage-based active material (Day3)| Construct cathode with phage-based active material]]<br>
 
| <font color= #01A9DB>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M3D3| Homework due]]
 
| <font color= #01A9DB>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M3D3| Homework due]]
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| R/F Apr/May 30/1
| [http://be.mit.edu/directory/angela-belcher AB]<br>
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| [http://be.mit.edu/directory/leslie-mcclain LM]<br>
 
| [[20.109(S20):Visualize phage nanowires using TEM (Day4)| Visualize phage nanowires using transmission electron microscopy]]<br>
 
| [[20.109(S20):Visualize phage nanowires using TEM (Day4)| Visualize phage nanowires using transmission electron microscopy]]<br>
 
| [[20.109(S20):Homework#Due_M3D4| Homework due]]
 
| [[20.109(S20):Homework#Due_M3D4| Homework due]]
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| T/W May 5/6
| [http://be.mit.edu/directory/angela-belcher AB]
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| [http://be.mit.edu/directory/leslie-mcclain LM]
 
| [[20.109(S20):Assemble and test batteries (Day5) | Assemble and test batteries ]]<br>
 
| [[20.109(S20):Assemble and test batteries (Day5) | Assemble and test batteries ]]<br>
 
| <font color= #01A9DB>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M3D5| Homework due]]
 
| <font color= #01A9DB>'''Laboratory quiz'''</font color> <br> [[20.109(S20):Homework#Due_M3D5| Homework due]]

Revision as of 19:07, 15 January 2020

20.109(S20): Laboratory Fundamentals of Biological Engineering

Sp20 banner image v2.png

Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (32-124)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
T/W Feb 4/5 NLL
Orientation and laboratory tour
1 1 R/F Feb 6/7 AK
In silico cloning and confirmation digest of protein expression vector
Orientation quiz
Homework due
1 2 T/W Feb 11/12 AK
Purify protein for secondary assays
Homework due
1 3 R/F Feb 13/14 AK
Analyze small molecule microarray (SMM) data
Homework due
T/W Feb 18/19 President's day holiday
1 4 R/F Feb 20/21 AK
Evaluate protein purity and concentration
Laboratory quiz
Homework due
1 5 T/W Feb 25/26 AK
Test protein activity using peptidyl-prolyl cis-trans isomerase assay
Homework due
1 6 R/F Feb 27/28 AK
Confirm ligand binding using differential scanning fluorimetry assay
Homework due
1 7 T/W Mar 3/4 AK
Complete data analysis
Laboratory quiz
Homework due
2 1 R/F Mar 5/6 NLL
Practice tissue culture techniques and prepare cells for RNA purification
Homework due
2 2 T/W Mar 10/11 EF
Etoposide treat cells for RNA purification
Homework due
Data summary draft due Mon, Mar 11 at 10 pm
2 3 R/F Mar 12/13 EF
Purify RNA and practice RNA-seq data analysis methods
Homework due
Mini-presentation due Sat, Mar 16 at 10 pm
[https:// Blog post due] Sun, Mar 17 at 10 pm
2 4 T/W Mar 17/18 EF
Analyze RNA-seq data and prepare for quantitative PCR experiment
Laboratory quiz
Homework due
2 5 R/F Mar 19/20 EF
Perform quantitative PCR experiment and explore additional RNA-seq datasets
Homework due
T/W Mar 24/25 Spring vacation Data summary revision due Mon, Mar 25 at 10 pm
R/F Mar 26/27 Spring vacation
2 6 T/W Mar/Apr 31/1 EF
Design cell viability experiment and complete RNA-seq data analysis
Homework due
2 7 R/F Apr 2/3 EF
Journal club I presentations
[https:// Blog post due] Sat, Apr 6 at 10 pm
2 8 T/W Apr 7/8 NLL
Journal club II presentations
3 1 R/F Apr 9/10 LM
Complete data analysis
Laboratory quiz
Homework due
3 2 R/F Apr 14/15 Comm Lab
Grow phage-based active (cathode) material
Homework due
Research article due Sat, Apr 20 at 10 pm
[https:// Blog post due] Sun, Apr 21 at 10 pm
3 3 T/W Apr 16/17 LM
Purify phage-based active (cathode) material
Homework due
T/W Apr 21/22 Patriots' day holiday
3 4 T/W Apr 23/24 LM
Construct cathode with phage-based active material
Laboratory quiz
Homework due
T/W Apr 28/29 Research proposal pitches in lecture, no laboratory
3 5 R/F Apr/May 30/1 LM
Visualize phage nanowires using transmission electron microscopy
Homework due
3 6 T/W May 5/6 LM Assemble and test batteries
Laboratory quiz
Homework due
R/F May 7/8 Research proposal presentations [https:// Blog post due] Sat, May 11 at 10 pm
T May 12 Feedback and celebratory lunch Mini-report due Mon, May 13 at 10 pm