20.109(S20):Module 3

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20.109(S20): Laboratory Fundamentals of Biological Engineering

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Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Module 3

Lecturer: Leslie McClain
Instructors: Noreen Lyell and Leslie McClain, and Becky Meyer
Research assistant: Sarah Cowles
TAs: Kevin Chung and Joe Kreitz
Lab manager: Hsinhwa Lee

Overview

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Lab links: day by day

M3D1: Enrich candidate clones using fluorescence-activated cell sorter (FACS)
M3D2: Harvest candidate clones and prepare for sequencing
M3D3: Identify clones to characterize
M3D4: Characterize clone by titration using flow cytometry
M3D5: Analyze titration curves

Assignments

Research proposal presentations
Mini-report

References

Notes for teaching faculty

Prep notes for M3
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