Difference between revisions of "20.109(S20):Module 3"

From Course Wiki
Jump to: navigation, search
(Module 3)
(Lab links: day by day)
Line 21: Line 21:
 
==Lab links: day by day==
 
==Lab links: day by day==
 
M3D1: [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br>
 
M3D1: [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br>
M3D2: [[20.109(S20):Harvest candidate clones  (Day2) |Harvest candidate clones ]]<br>
+
M3D2: [[20.109(S20):Harvest candidate clones  (Day2) |Harvest candidate clones and prepare for sequencing  ]]<br>
M3D3: [[20.109(S20):Prepare clones for sequencing  (Day3) |Prepare clones for sequencing  ]]<br>
+
M3D3: [[20.109(S20):Identify clones to characterize  (Day3) |Identify clones to characterize]]<br>
M3D4: [[20.109(S20):Identify clone to characterize  (Day4)|Identify clone to characterize ]]<br>
+
M3D4: [[20.109(S20):Characterize clone by titration using flow cytometry (Day4)|Characterize clone by titration using flow cytometry ]]<br>
M3D5: [[20.109(S20):Characterize clone by titration using flow cytometry (Day5)|Characterize clone by titration using flow cytometry ]]<br>
+
M3D5: [[20.109(S20):Analyze titration curves (Day5) |Analyze titration curves ]]<br>
M3D6: [[20.109(S20):Analyze titration curves (Day6) |Analyze titration curves ]]<br>
+
  
 
==Assignments==
 
==Assignments==

Revision as of 19:12, 17 March 2020

20.109(S20): Laboratory Fundamentals of Biological Engineering

Sp20 banner image v2.png

Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Module 3

Lecturer: Leslie McClain
Instructors: Noreen Lyell and Leslie McClain, and Becky Meyer
Research assistant: Sarah Cowles
TAs: Kevin Chung and Joe Kreitz
Lab manager: Hsinhwa Lee

Overview

[[|thumb|775px|center|]]


Lab links: day by day

M3D1: Enrich candidate clones using fluorescence-activated cell sorter (FACS)
M3D2: Harvest candidate clones and prepare for sequencing
M3D3: Identify clones to characterize
M3D4: Characterize clone by titration using flow cytometry
M3D5: Analyze titration curves

Assignments

Research proposal presentations
Mini-report

References

Notes for teaching faculty

Prep notes for M3