Difference between revisions of "20.109(S20):Module 3"

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(Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #0489B1;"> {{Template:20.109(S20)}} =<center>Module 3</center>= '''Lecturer:''' [http://be.mit.edu/directory/le...")
 
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=<center>Module 3</center>=
 
=<center>Module 3</center>=
  
'''Lecturer:''' [http://be.mit.edu/directory/leslie-mcclain Leslie McClain]  
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'''Lecturer:''' [http://be.mit.edu/directory/leslie-mcclain Leslie McClain] <br>
 
'''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell] and [http://be.mit.edu/directory/leslie-mcclain Leslie McClain], and [http://be.mit.edu/directory/becky-meyer Becky Meyer]<br>
 
'''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell] and [http://be.mit.edu/directory/leslie-mcclain Leslie McClain], and [http://be.mit.edu/directory/becky-meyer Becky Meyer]<br>
 
'''Research assistant:''' ??? <br>
 
'''Research assistant:''' ??? <br>

Revision as of 17:09, 23 January 2020

20.109(S20): Laboratory Fundamentals of Biological Engineering

Sp20 banner image v2.png

Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Module 3

Lecturer: Leslie McClain
Instructors: Noreen Lyell and Leslie McClain, and Becky Meyer
Research assistant: ???
TAs: Kevin Chung and Joe Kreitz
Lab manager: Hsinhwa Lee

Overview

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Lab links: day by day

M3D1: Enrich candidate clones using fluorescence-activated cell sorter (FACS)
M3D2: Harvest candidate clones
M3D3: Prepare clones for sequencing
M3D4: Identify clone to characterize
M3D5: Characterize clone by titration using flow cytometry
M3D6: Analyze titration curves

Assignments

Research proposal presentations
Mini-report

References

Notes for teaching faculty

Prep notes for M3