20.109(S20):Module 2

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20.109(S20): Laboratory Fundamentals of Biological Engineering

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Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Module 2

Lecturers: Ernest Fraenkel and Noreen Lyell
Instructors: Noreen Lyell and Leslie McClain, and Becky Meyer Research assistant: Anne Shen TAs: Kevin Chung and Joe Kreitz
Lab manager: Hsinhwa Lee

Overview

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Lab links: day by day

M2D1: Prepare cells for RNA purification and practice RNA-seq data analysis methods
M2D2: Treat cells with etoposide and analyze RNA-seq data
M2D3: Purify RNA from etoposide-treated cells and generate cDNA
M2D4: Journal club I presentations
M2D5: Journal club II presentations
M2D6: Analyze RNA-seq data and select gene targets for quantitative PCR experiment
M2D7: Perform qPCR experiment and continue RNA-seq data analysis
M2D8: Examine qPCR results and complete data analysis

Assignments

Journal club presentation
Research article

References

Notes for teaching faculty

Prep notes for M2
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