20.109(S20):Examine qPCR results and complete data analysis (Day8)

Introduction

Throughout Module 2 you have used two datasets to study the effect of etoposide treatment on cancer cell lines (DLD-1 and A549). In the previous exercises you examined gene expression in each cell line. Today you will use the skills learned to compare gene expression across DLD-1 and A549.

As an introduction to how you will discuss and interpret the data in your Research article, let's work though the below image:

Heatmap comparing the Fisher's statistic of the top 100 significantly enriched GO terms across the DLD-1 and A549 datasets.

Protocols

Part 1: Analyze RNA-seq data

Today you will complete your analysis of the RNA-seq data sets!

Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, compare the RNA-seq results for DLD-1 and A549 to identify similarities and / or differences in gene expression that exist when cells are treated with etoposide.

Navigation links

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