20.109(S20):Examine qPCR results and complete data analysis (Day8)

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20.109(S20): Laboratory Fundamentals of Biological Engineering

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Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Introduction

Heatmap comparing the Fisher's statistic of the top 100 significantly enriched GO terms across the DLD-1 and A549 datasets.

Protocols

Part 1: Analyze RNA-seq data

Today you will complete your analysis of the RNA-seq data sets!

Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, compare the RNA-seq results for DLD-1 and A549 to identify similarities and / or differences in gene expression that exist when cells are treated with etoposide.

Navigation links

Next day: Enrich candidate clones using fluorescence-activated cell sorter (FACS)

Previous day: Review qPCR experiment and complete statistical analysis
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