Difference between revisions of "20.109(S20):Examine qPCR results and complete data analysis (Day8)"
From Course Wiki
Noreen Lyell (Talk | contribs) (→Part 1: Analyze RNA-seq data) |
Noreen Lyell (Talk | contribs) (→Part 1: Analyze RNA-seq data) |
||
Line 10: | Line 10: | ||
Today you will complete your analysis of the RNA-seq data sets! | Today you will complete your analysis of the RNA-seq data sets! | ||
− | Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, compare the | + | Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, compare the transcription profiles of DLD-1 and A549 to identify similarities and / or differences in gene expression that exist when cells are treated with etoposide. |
==Navigation links== | ==Navigation links== | ||
Next day: [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br> | Next day: [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br> | ||
Previous day: [[20.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7) | Review qPCR experiment and complete statistical analysis]]<br> | Previous day: [[20.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7) | Review qPCR experiment and complete statistical analysis]]<br> |
Revision as of 17:09, 18 March 2020
Introduction
Protocols
Part 1: Analyze RNA-seq data
Today you will complete your analysis of the RNA-seq data sets!
Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, compare the transcription profiles of DLD-1 and A549 to identify similarities and / or differences in gene expression that exist when cells are treated with etoposide.
Next day: Enrich candidate clones using fluorescence-activated cell sorter (FACS)