Difference between revisions of "20.109(S20):Examine qPCR results and complete data analysis (Day8)"

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Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel.  In this exercise, compare the gene expression profiles of DLD-1 and A549 to identify similarities that may, or may not, exist when cells are treated with etoposide.
 
Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel.  In this exercise, compare the gene expression profiles of DLD-1 and A549 to identify similarities that may, or may not, exist when cells are treated with etoposide.
 
==Reagents list==
 
  
 
==Navigation links==
 
==Navigation links==
 
Next day: [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br>
 
Next day: [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br>
 
Previous day: [[20.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7) | Review qPCR experiment and complete statistical analysis]]<br>
 
Previous day: [[20.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7) | Review qPCR experiment and complete statistical analysis]]<br>

Revision as of 17:05, 18 March 2020

20.109(S20): Laboratory Fundamentals of Biological Engineering

Sp20 banner image v2.png

Spring 2020 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Screening ligand binding        2. Measuring gene expression        3. Engineering antibodies              


Introduction

Protocols

Part 1: Analyze RNA-seq data

Today you will complete your analysis of the RNA-seq data!

Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, compare the gene expression profiles of DLD-1 and A549 to identify similarities that may, or may not, exist when cells are treated with etoposide.

Navigation links

Next day: Enrich candidate clones using fluorescence-activated cell sorter (FACS)

Previous day: Review qPCR experiment and complete statistical analysis