Difference between revisions of "20.109(S20):Examine qPCR results and complete data analysis (Day8)"
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Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, compare the gene expression profiles of DLD-1 and A549 to identify similarities that may, or may not, exist when cells are treated with etoposide. | Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, compare the gene expression profiles of DLD-1 and A549 to identify similarities that may, or may not, exist when cells are treated with etoposide. | ||
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==Navigation links== | ==Navigation links== | ||
Next day: [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br> | Next day: [[20.109(S20):Enrich candidate clones using fluorescence-activated cell sorter (FACS) (Day1)| Enrich candidate clones using fluorescence-activated cell sorter (FACS)]]<br> | ||
Previous day: [[20.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7) | Review qPCR experiment and complete statistical analysis]]<br> | Previous day: [[20.109(S20):Perform qPCR experiment and continue RNA-seq data analysis (Day7) | Review qPCR experiment and complete statistical analysis]]<br> |
Revision as of 17:05, 18 March 2020
Introduction
Protocols
Part 1: Analyze RNA-seq data
Today you will complete your analysis of the RNA-seq data!
Complete Exercise #4 developed by Amanda Kedaigle, Anne Shen & Prof. Ernest Fraenkel. In this exercise, compare the gene expression profiles of DLD-1 and A549 to identify similarities that may, or may not, exist when cells are treated with etoposide.
Next day: Enrich candidate clones using fluorescence-activated cell sorter (FACS)