Difference between revisions of "20.109(F19): Fall 2019 schedule"

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(Created page with "<div style="padding: 10px; width: 820px; border: 5px solid #A4A4A4;"> {{Template:20.109(F19)}} Welcome to 20.109! It is our goal to make this class a useful and fun introduc...")
 
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| R/F Sept 6/7  
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| R/F Sept 5/6  
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [[Media:Fa18 Orientation lecture.pdf| Orientation lecture]]
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| [http://be.mit.edu/directory/noreen-lyell NLL]<br>  
| [[20.109(F18):Lab tour | Orientation]]<br>[[Media:M0D0_Fa18_JBmarkded.pdf| Prelab T/R]]<br>[[Media:Fa18_MOD0_LMMmarked01.pdf | Prelab W/F]]
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| [[20.109(F19):Lab tour | Orientation]]<br>
 
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| 1
 
| 1
 
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| T/W Sept 11/12
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| T/W Sept 10/11
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F18):Practice tissue culture and prepare microwell array (Day1)| Practice tissue culture and prepare microwell array]]<br>[[Media:Fa18__M1D1_JBmarked.pdf| Prelab T/R]]<br>[[Media:M1D1_Fa18_LM_marked.pdf | Prelab W/F]]
+
| [[20.109(F19):Practice tissue culture and prepare microwell array (Day1)| Practice tissue culture and prepare microwell array]]<br>
| <font color=#FF0000>'''Laboratory orientation quiz'''</font color><br>[[20.109(F18):Homework#Due_M1D1|Homework due]]
+
| <font color=#FF0000>'''Laboratory orientation quiz'''</font color><br>[[20.109(F19):Homework#Due_M1D1|Homework due]]
 
|--
 
|--
 
| 1
 
| 1
 
| 2
 
| 2
| R/F Sept 13/14    
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| R/F Sept 12/13    
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F18):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)| Design cell loading optimization experiment and research cell lines]]<br>[[Media:Fa18__M1D2_JBmarked.pdf| Prelab T/R]]<br>[[Media:M1D2_Fa18_LM.pdf| Prelab W/F]]
+
| [[20.109(F19):Design cell loading optimization experiment and discuss genomic instability experiment (Day2)| Design cell loading optimization experiment and research cell lines]]<br>
| [[20.109(F18):Homework#Due_M1D2|Homework due]]
+
| [[20.109(F19):Homework#Due_M1D2|Homework due]]
 
|--
 
|--
 
| 1
 
| 1
 
| 3
 
| 3
| T/W Sept 18/19  
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| T/W Sept 17/18  
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F18):Prepare and treat cells for genomic instability experiment (Day3)| Prepare and treat cells for genomic instability experiment]]<br>[[Media:Fa18__M1D3_JBmarked.pdf| Prelab T/R]]<br>[[Media:M1D3_Fa18_LMmarked.pdf| Prelab W/F]]
+
| [[20.109(F19):Prepare and treat cells for genomic instability experiment (Day3)| Prepare and treat cells for genomic instability experiment]]<br>
| [[20.109(F18):Homework#Due_M1D3|Homework due]]
+
| [[20.109(F19):Homework#Due_M1D3|Homework due]]
 
|--
 
|--
 
|  
 
|  
 
|  
 
|  
| R/F Sept 20/21
+
| R/F Sept 19/20
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| <font color = #33ff5e>'''Lecture, but no laboratory'''</font color><br><font color = 999999>Career fair student holiday</font color><br>
 
| <font color = #33ff5e>'''Lecture, but no laboratory'''</font color><br><font color = 999999>Career fair student holiday</font color><br>
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| 1
 
| 1
 
| 4
 
| 4
| T/W Sept 25/26
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| T/W Sept 24/25
 
| Comm Lab
 
| Comm Lab
| [[20.109(F18):Complete genomic instability experiment and load cells for sub-nuclear foci assay (Day4)| Complete genomic instability experiment and load cells for sub-nuclear foci assay]]<br>[[Media:Fa18__M1D4_JBmarked.pdf| Prelab T/R]]<br>[[Media:M1D4_Fa18_LMmarked.pdf| Prelab W/F]]
+
| [[20.109(F19):Complete genomic instability experiment and load cells for sub-nuclear foci assay (Day4)| Complete genomic instability experiment and load cells for sub-nuclear foci assay]]<br>
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F18):Homework#Due_M1D4|Homework due]]
+
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M1D4|Homework due]]
 
|--
 
|--
 
| 1
 
| 1
 
| 5
 
| 5
| R/F Sept 27/28
+
| R/F Sept 26/27
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F18):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)| Analyze instability experiment data and treat cells for sub-nuclear foci assay]]<br>[[Media:Fa18__M1D5_JBmarked.pdf| Prelab T/R]]<br>[[Media:M1D5_Fa18_LMmarked.pdf| Prelab W/F]]
+
| [[20.109(F19):Analyze genomic instability experiment data and treat cells for sub-nuclear foci assay (Day5)| Analyze instability experiment data and treat cells for sub-nuclear foci assay]]<br>
| [[20.109(F18):Homework#Due_M1D5|Homework due]]
+
| [[20.109(F19):Homework#Due_M1D5|Homework due]]
 
|--
 
|--
 
| 1
 
| 1
 
| 6
 
| 6
| T/W Oct 2/3
+
| T/W Oct 1/2
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F18):Complete sub-nuclear foci assay (Day6)| Complete sub-nuclear foci assay]]<br>[[Media:Fa18_M1D6_JBmarked.pdf| Prelab T/R]]<br>[[Media:M1D6_Fa18_LMmarked.pdf| Prelab W/F]]
+
| [[20.109(F19):Complete sub-nuclear foci assay (Day6)| Complete sub-nuclear foci assay]]<br>
| [[20.109(F18):Homework#Due_M1D6|Homework due]]
+
| [[20.109(F19):Homework#Due_M1D6|Homework due]]
 
|--
 
|--
 
| 1
 
| 1
 
| 7
 
| 7
| R/F Oct 4/5
+
| R/F Oct 3/4
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
 
| [http://be.mit.edu/directory/bevin-p-engelward BE]
| [[20.109(F18):Practice statistical analysis methods and complete data analysis (Day7)| Practice statistical analysis methods and complete data analysis]]<br>[[Media:Fa18_M1D7_JBmarked.pdf| Prelab T/R]]<br>[[Media:M1D7_Fa18_LMmarked.pdf| Prelab W/F]]
+
| [[20.109(F19):Practice statistical analysis methods and complete data analysis (Day7)| Practice statistical analysis methods and complete data analysis]]<br>
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F18):Homework#Due_M1D7|Homework due]]
+
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M1D7|Homework due]]
 +
|--
 +
| 2
 +
| 1
 +
| R/F Oct 8/9
 +
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 +
| [[20.109(F19):Complete in silico cloning (Day1)| Complete in silico cloning]]<br>
 +
| [[20.109(F19):Homework#Due_M2D1| Homework due]]<br>[[20.109(F19): Mini-presentation| Mini-presentation due]] Sat, X at 10 pm
 +
|--
 +
| 2
 +
| 2
 +
| T/W Oct 10/11
 +
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
 +
| [[20.109(F19):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br>
 +
| [[20.109(F19):Homework#Due_M2D2|Homework due]]
 
|--
 
|--
 
|  
 
|  
 
|  
 
|  
| <font color = 999999>T/W Oct 9/10</font color>
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| <font color = 999999>T/W Oct 15/16</font color>
 
|  
 
|  
 
| <font color = 999999>Columbus day holiday</font color>
 
| <font color = 999999>Columbus day holiday</font color>
| [[20.109(F18): Data Summary| Data Summary draft due]] Wed, Oct 10 at 10 pm<br>[https://be20109f18.blogspot.com/ Blog post due] Thurs, Oct 11 at 10 pm
+
| [[20.109(F19): Data Summary| Data Summary draft due]] Wed, X at 10 pm<br>[ Blog post due] Thurs, X at 10 pm
 
|--
 
|--
 
| colspan="7" |
 
| colspan="7" |
|--
 
| 2
 
| 1
 
| R/F Oct 11/12
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 m2d1 lecture slides.pdf| M2D1 lecture]]
 
| [[20.109(F18):Complete in silico cloning (Day1)| Complete in silico cloning]]<br>[[Media:Fa18_M2D1_JBmarked.pdf| Prelab T/R]]<br>[[Media:M2D1_Fa18_LMmarked.pdf | Prelab W/F]]
 
| [[20.109(F18):Homework#Due_M2D1| Homework due]]<br>[[20.109(F18): Mini-presentation| Mini-presentation due]] Sat, Oct 13 at 10 pm
 
|--
 
| 2
 
| 2
 
| T/W Oct 16/17
 
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D2 lecture slides.pdf| M2D2 lecture]]
 
| [[20.109(F18):Design gRNA for CRISPRi (Day2)| Design gRNA for CRISPRi]]<br>[[Media:Fa18_M2D2_JBmarked.pdf| Prelab T/R]]<br>[[Media:M2D2_Fa18_LMmarked.pdf | Prelab W/F]]
 
| [[20.109(F18):Homework#Due_M2D2|Homework due]]
 
 
|--
 
|--
 
| 2
 
| 2
 
| 3
 
| 3
| R/F Oct 18/19
+
| R/F Oct 17/18
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D3 figures.pptx| Paper figures]]<br>[[Media:Fa18 M2D3 lecture slides.pdf| M2D3 slides]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F18):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br>[[Media:Fa18_M2D3_JBmarked.pdf| Prelab T/R]]<br>[[Media:M2D3_Fa18_LMmarked.pdf| Prelab W/F]]<br>
+
| [[20.109(F19):Generate gRNA plasmid (Day3)| Generate gRNA plasmid]]<br>
| [[20.109(F18):Homework#Due_M2D3|Homework due]]<br>[[20.109(F18): Data Summary| Data Summary revision due]] Sat, Oct 20 at 10 pm
+
| [[20.109(F19):Homework#Due_M2D3|Homework due]]<br>[[20.109(F19): Data Summary| Data Summary revision due]] Sat, X at 10 pm
 
|--
 
|--
 
| 2
 
| 2
 
| 4
 
| 4
| T/W Oct 23/24
+
| T/W Oct 22/23
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D4 lecture slides students.pdf| M2D4 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F18):Journal club presentation (Day4 and 6)| Journal club I]]
+
| [[20.109(F19):Journal club presentation (Day4 and 6)| Journal club I]]
 
|  
 
|  
 
|--
 
|--
 
| 2
 
| 2
 
| 5
 
| 5
| R/F Oct 25/26
+
| R/F Oct 24/25
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D5 lecture slides.pdf| M2D5 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F18):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br>[[Media:Fa18_M2D5_JBmarked.pdf| Prelab T/R]]<br>[[Media:M2D5_Fa18_LMmarked.pdf| Prelab W/F]]<br>
+
| [[20.109(F19):Confirm gRNA sequence (Day5)| Confirm gRNA sequence]]<br>
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F18):Homework#Due_M2D5|Homework due]]
+
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M2D5|Homework due]]
 
|--
 
|--
 
| 2
 
| 2
 
| 6
 
| 6
| T/W Oct 30/31
+
| T/W Oct 29/30
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D6 lecture slides.pdf| M2D6 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F18):Journal club presentation (Day4 and 6) | Journal club II]]
+
| [[20.109(F19):Journal club presentation (Day4 and 6) | Journal club II]]
 
|  
 
|  
 
|--
 
|--
 
| 2
 
| 2
 
| 7
 
| 7
| R/F Nov 1/2
+
| R/F Oct/Nov 31/1
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D7 lecture slides.pdf| M2D7 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F18):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br>[[Media:Fa18_M2D7_JBmarked.pdf| Prelab T/R]]<br>[[Media:Fa18_M2D7_JPmarked.pdf| Prelab W/F]]<br>
+
| [[20.109(F19):Induce CRISPRi system (Day7)| Induce CRISPRi system]]<br>
| [[20.109(F18):Homework#Due_M2D7|Homework due]]<br>[https://be20109f18.blogspot.com/ Blog post due] Fri, Nov 2 at 10 pm
+
| [[20.109(F19):Homework#Due_M2D7|Homework due]]<br>[ Blog post due] Fri, X at 10 pm
 
|--
 
|--
 
| 2
 
| 2
 
| 8
 
| 8
| T/W Nov 6/7
+
| T/W Nov 5/6
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>[[Media:Fa18 M2D8 lecture slides.pdf| M2D8 lecture]]
+
| [http://be.mit.edu/directory/noreen-lyell NLL]<br>
| [[20.109(F18):Measure_fermentation_products_(Day8) | Measure fermentation products]]<br>[[Media:Fa18_M2D8_JBmarked.pdf| Prelab T/R]]<br>[[Media:Fa18_M2D8_LMmarked.pdf| Prelab W/F]]
+
| [[20.109(F19):Measure_fermentation_products_(Day8) | Measure fermentation products]]<br>
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F18):Homework#Due_M2D8|Homework due]]
+
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M2D8|Homework due]]
 
|--
 
|--
 
| colspan="7" |
 
| colspan="7" |
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| 1
 
| 1
 
| R/F Nov 8/9
 
| R/F Nov 8/9
| [http://be.mit.edu/directory/angela-belcher AB]  
+
|   
| [[20.109(F18):Growth of phage-based active (cathode) material (Day1) | Grow phage-based active (cathode) material]]<br>[[Media:Fa18_M3D1_JBmarked.pdf| Prelab T/R]]<br>[[Media: M3D1_Fa18_LMmarked.pdf | Prelab W/F]]
+
|  
| [[20.109(F18):Homework#Due_M3D1|Homework due]] <br>  
+
| [[20.109(F19):Homework#Due_M3D1|Homework due]] <br>  
 
|--
 
|--
 
|  
 
|  
 
|
 
|
| <font color = 999999>T/W Nov 13/14</font color>
+
| <font color = 999999>T/W Nov 12/13</font color>
 
|  
 
|  
 
| <font color = 999999>Veterans' day holiday</font color>
 
| <font color = 999999>Veterans' day holiday</font color>
| [[20.109(F18): Research article| Research article due]]  Mon, Nov 12 at 10 pm <br>[https://be20109f18.blogspot.com/ Blog post due] Tue, Nov 13 at 10 pm
+
| [[20.109(F19): Research article| Research article due]]  Mon, X at 10 pm <br>[ Blog post due] Tue, X at 10 pm
 
|--
 
|--
 
| 3
 
| 3
 
| 2
 
| 2
| R/F Nov 15/16
+
| R/F Nov 14/15
| [http://be.mit.edu/directory/angela-belcher AB] 
+
|  
| [[20.109(F18):Purify phage-based active (cathode) material (Day2) | Purify phage-based active (cathode) material]]<br>[[Media:Fa18_M3D2_JBmarked.pdf| Prelab T/R]]<br>[[Media:M3D2_Fa18_LMmarked.pdf| Prelab W/F]]
+
|  
| [[20.109(F18):Homework#Due_M3D2|Homework due]]<br>
+
| [[20.109(F19):Homework#Due_M3D2|Homework due]]<br>
 
|--
 
|--
 
|
 
|
 
|
 
|
| T/W Nov 20/21
+
| T/W Nov 19/20
| [http://be.mit.edu/directory/angela-belcher AB]<br>
+
|  
 
| <font color = #33ff5e>'''Lecture, but no laboratory'''</font color>
 
| <font color = #33ff5e>'''Lecture, but no laboratory'''</font color>
 
|  
 
|  
Line 179: Line 179:
 
|
 
|
 
|
 
|
| <font color = 999999>R/F Nov 22/23</font color>
+
| <font color = 999999>R/F Nov 21/22</font color>
 
|
 
|
 
| <font color = 999999>Thanksgiving holiday</font color>
 
| <font color = 999999>Thanksgiving holiday</font color>
Line 186: Line 186:
 
| 3
 
| 3
 
| 3
 
| 3
| T/W Nov 27/28
+
| T/W Nov 26/27
| [http://be.mit.edu/directory/angela-belcher AB] <br>[[Media:BATTERIES_20.109_2018.pdf | M3D4 lecture]]
+
|  
| [[20.109(F18):Construct cathode with phage-based active material (Day3)| Construct cathode with phage-based active material]]<br>[[Media:Fa18_M3D3_JBmarked.pdf| Prelab T/R]]<br>[[Media:M3D3_Fa18_LM.pdf| Prelab W/F]]
+
|  
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F18):Homework#Due_M3D3| Homework due]]
+
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M3D3| Homework due]]
 
|--
 
|--
 
| 3
 
| 3
 
| 4
 
| 4
| R/F Nov/Dec 29/30
+
| R/F Nov 28/29
| [http://be.mit.edu/directory/angela-belcher AB] <br>[[Media: 20.109_TEM_2018_FALL.pdf | M3D4 lecture]]
+
|  
| [[20.109(F18):Visualize phage nanowires using TEM (Day4)| Visualize phage nanowires using transmission electron microscopy]]<br>[[Media:Fa18_M3D4_CSmarked.pdf| Prelab T/R]]<br>[[Media:M3D4_Fa18_LMmarked.pdf| Prelab W/F]]
+
|  
| [[20.109(F18):Homework#Due_M3D4|Homework due]]
+
| [[20.109(F19):Homework#Due_M3D4|Homework due]]
 
|--
 
|--
 
| 3
 
| 3
 
| 5
 
| 5
| T/W Dec 4/5  
+
| T/W Dec 3/4   
| [http://be.mit.edu/directory/angela-belcher AB]<br>
+
|  
| [[20.109(F18):Assemble and test batteries (Day5) | Assemble and test batteries ]]<br>[[Media:Fa18_M3D5_JBmarked.pdf| Prelab T/R]]<br>
+
|  
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F18):Homework#Due_M3D5|Homework due]]
+
| <font color=#FF0000>'''Laboratory quiz'''</font color><br>[[20.109(F19):Homework#Due_M3D5|Homework due]]
 
|--
 
|--
 
|  
 
|  
 
|  
 
|  
| R/F Dec 6/7
+
| R/F Dec 5/6
 
|  
 
|  
| [[20.109(F18):Research proposal presentation| Research proposal presentations]]
+
| [[20.109(F19):Research proposal presentation| Research proposal presentations]]
| [[20.109(F18):Research proposal presentation| Research proposal presentation due]] Thu/Fri, Dec 6/7 at 1 pm<br>[https://be20109f18.blogspot.com/ Blog post due] Fri, Dec 7 at 10 pm
+
| [ Blog post due] Fri, X at 10 pm
 
|--
 
|--
 
| colspan="7" |
 
| colspan="7" |
Line 216: Line 216:
 
|  
 
|  
 
|  
 
|  
| T Dec 11
+
| T Dec 10
 
|  
 
|  
 
| <font color = #33ff5e>'''Feedback and celebratory lunch'''</font color>
 
| <font color = #33ff5e>'''Feedback and celebratory lunch'''</font color>
| [[20.109(F18): Mini-report | Mini-report due]] Mon, Dec 10 at 10 pm <br>
+
| [[20.109(F19): Mini-report | Mini-report due]] Mon, Dec 9 at 10 pm <br>
 
|}
 
|}
 
</div>
 
</div>

Revision as of 19:04, 13 June 2019

20.109(F19): Laboratory Fundamentals of Biological Engineering

Fa19 20109 Banner image.png

Fall 2019 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Measuring genomic instability        2. Modulating metabolism        3. Testing chemical probes              


Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.

SCHEDULE DETAILS:
Lecture times: T/R 11 - 12 pm (16-220)
Laboratory section times: T/R 1 - 5 pm or W/F 1 - 5 pm (56-322)

MODULE DAY DATE LECTURER LABORATORY EXPERIMENTS ASSIGNMENTS
R/F Sept 5/6 NLL
Orientation
1 1 T/W Sept 10/11 BE Practice tissue culture and prepare microwell array
Laboratory orientation quiz
Homework due
1 2 R/F Sept 12/13 BE Design cell loading optimization experiment and research cell lines
Homework due
1 3 T/W Sept 17/18 BE Prepare and treat cells for genomic instability experiment
Homework due
R/F Sept 19/20 BE Lecture, but no laboratory
Career fair student holiday
1 4 T/W Sept 24/25 Comm Lab Complete genomic instability experiment and load cells for sub-nuclear foci assay
Laboratory quiz
Homework due
1 5 R/F Sept 26/27 BE Analyze instability experiment data and treat cells for sub-nuclear foci assay
Homework due
1 6 T/W Oct 1/2 BE Complete sub-nuclear foci assay
Homework due
1 7 R/F Oct 3/4 BE Practice statistical analysis methods and complete data analysis
Laboratory quiz
Homework due
2 1 R/F Oct 8/9 NLL
Complete in silico cloning
Homework due
Mini-presentation due Sat, X at 10 pm
2 2 T/W Oct 10/11 NLL
Design gRNA for CRISPRi
Homework due
T/W Oct 15/16 Columbus day holiday Data Summary draft due Wed, X at 10 pm
[ Blog post due] Thurs, X at 10 pm
2 3 R/F Oct 17/18 NLL
Generate gRNA plasmid
Homework due
Data Summary revision due Sat, X at 10 pm
2 4 T/W Oct 22/23 NLL
Journal club I
2 5 R/F Oct 24/25 NLL
Confirm gRNA sequence
Laboratory quiz
Homework due
2 6 T/W Oct 29/30 NLL
Journal club II
2 7 R/F Oct/Nov 31/1 NLL
Induce CRISPRi system
Homework due
[ Blog post due] Fri, X at 10 pm
2 8 T/W Nov 5/6 NLL
Measure fermentation products
Laboratory quiz
Homework due
3 1 R/F Nov 8/9 Homework due
T/W Nov 12/13 Veterans' day holiday Research article due Mon, X at 10 pm
[ Blog post due] Tue, X at 10 pm
3 2 R/F Nov 14/15 Homework due
T/W Nov 19/20 Lecture, but no laboratory
R/F Nov 21/22 Thanksgiving holiday
3 3 T/W Nov 26/27 Laboratory quiz
Homework due
3 4 R/F Nov 28/29 Homework due
3 5 T/W Dec 3/4 Laboratory quiz
Homework due
R/F Dec 5/6 Research proposal presentations [ Blog post due] Fri, X at 10 pm
T Dec 10 Feedback and celebratory lunch Mini-report due Mon, Dec 9 at 10 pm