20.109(F19):Module 1

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20.109(F19): Laboratory Fundamentals of Biological Engineering

Fa19 20109 Banner image.png

Fall 2019 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Measuring genomic instability        2. Modulating metabolism        3. Testing chemical probes              


Module 1

Lecturer: Bevin Engelward
Instructors: Noreen Lyell, Leslie McClain and Becky Meyer
TAs: Colin Kim and Shelbi Parker
Lab manager: Hsinhwa Lee

Overview

Lab links: day by day

M1D1: Practice cell culture and begin gamma-H2AX assay
M1D2: Treat cells for gamma-H2AX assay and prepare CometChip for loading experiment
M1D3: Complete gamma-H2AX assay and complete loading experiment
M1D4: Load cells into CometChip and apply treatments for DNA damage experiment
M1D5: Complete DNA damage experiment
M1D6: Image CometChip
M1D7: Practice statistical analysis methods and complete data analysis

Assignments

Data summary
Mini-presentation

References

CometChip: A high-throughput 96-well platform for measuring DNA damage in microarrayed human cells. Journal of Visualized Experiments. (2014) 92: 1-11.

  • A video of the procedure is linked here.

CometChip: Single-cell microarray for high-throughput detection of DNA damage. Methods in Cell Biology. (2012) 112: 247-268.

Notes for teaching faculty

Prep notes for M1
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