Difference between revisions of "20.109(F19):Module 1"
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M1D1: [[20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)| Practice cell culture and begin gamma-H2AX assay]]<br> | M1D1: [[20.109(F19):Practice cell culture and begin sub-nuclear foci assay (Day1)| Practice cell culture and begin gamma-H2AX assay]]<br> | ||
M1D2: [[20.109(F19):Treat cells for gamma-H2AX (Day2)| Treat cells for gamma-H2AX assay and prepare CometChip for loading experiment]]<br> | M1D2: [[20.109(F19):Treat cells for gamma-H2AX (Day2)| Treat cells for gamma-H2AX assay and prepare CometChip for loading experiment]]<br> | ||
| − | M1D3: [[20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)| Complete gamma-H2AX assay and | + | M1D3: [[20.109(F19):Complete gamma-H2AX assay and prepare CometChip (Day3)| Complete gamma-H2AX assay and perform loading experiment]]<br> |
M1D4: [[20.109(F19):Load cells into CometChip and apply treatments (Day4)| Load cells into CometChip and apply treatments for DNA damage experiment]]<br> | M1D4: [[20.109(F19):Load cells into CometChip and apply treatments (Day4)| Load cells into CometChip and apply treatments for DNA damage experiment]]<br> | ||
M1D5: [[20.109(F19):Complete CometChip experiment (Day5)| Complete DNA damage experiment]]<br> | M1D5: [[20.109(F19):Complete CometChip experiment (Day5)| Complete DNA damage experiment]]<br> | ||
Revision as of 22:50, 20 August 2019
Contents
Module 1
Lecturer: Bevin Engelward
Instructors: Noreen Lyell, Leslie McClain and Becky Meyer
TAs: Colin Kim and Shelbi Parker
Lab manager: Hsinhwa Lee
Overview
Lab links: day by day
M1D1: Practice cell culture and begin gamma-H2AX assay
M1D2: Treat cells for gamma-H2AX assay and prepare CometChip for loading experiment
M1D3: Complete gamma-H2AX assay and perform loading experiment
M1D4: Load cells into CometChip and apply treatments for DNA damage experiment
M1D5: Complete DNA damage experiment
M1D6: Image CometChip
M1D7: Practice statistical analysis methods and complete data analysis
Assignments
Data summary
Mini-presentation
References
CometChip: A high-throughput 96-well platform for measuring DNA damage in microarrayed human cells. Journal of Visualized Experiments. (2014) 92: 1-11.
- A video of the procedure is linked here.
CometChip: Single-cell microarray for high-throughput detection of DNA damage. Methods in Cell Biology. (2012) 112: 247-268.
