20.109(F19):Begin Western blot analysis (Day3)

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20.109(F19): Laboratory Fundamentals of Biological Engineering

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Fall 2019 schedule        FYI        Assignments        Homework        Class data        Communication
       1. Measuring genomic instability        2. Modulating metabolism        3. Testing chemical probes              


Introduction

WESTERN BLOT

Protocols

Part 1: Lyse cells

Part 2: Electrophorese cellular proteins

Part 3: Transfer proteins to membrane

Reagents list

Navigation links

Next day: Complete antibody staining for Western blot analysis

Previous day: Incubate with ligand and apply heat treatment for protein denaturation
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