20.109(F17): Notes for M1

M1D1

Per group, aliquot

• 50 mL 1x PBS
• in TC room, 15 mL of “TK6 media”

M1D2

Per group, aliquot

• in main lab
  • 10 mL 1x PBS
  • 2 eppendorf tubes of 0.3% LMP agarose (in eppendorf water bath)
  • large scheme of the plate so they can keep track of how to load chips
• and separately, in the TC hood,
  • 10 mL 1x PBS
  • 25 mL TK6 media
  • 2 eppendorf tubes of 0.3% LMP agarose (in water bath at 43 °C)
  • 2x 15 mL of TK6 cells at 500,000 cells/mL

Set up hoods in TC room with, per group:

• 4 binder clips
• 1 glass slide
• 1 bottomless 96-well plate
• 1 razor blade
• Pasteur pipets
• sharp jar
• pipet aid
• pen
• team sticker to identify glass slides
• printout of today's protocol
• large scheme of the plate to hang in TC so they can keep track of how to load chips