20.109(F16):Module 2

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20.109(F16): Laboratory Fundamentals of Biological Engineering

Engelward PNAS 2006.png

Schedule Fall 2016        Announcements        Assignments        Homework        Communication
       1. Measuring Genomic Instability        2. Manipulating Metabolism        3. Engineering Biomaterials              

Module 2

Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas

TA:
Lab manager: Hsinhwa Lee

Overview

In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or acetate production is increased.


[[Image: |thumb|center|600px|Experimental overview for Module 2]]


Lab links: day by day

M1D1: Complete in silico cloning
M1D2: Design gRNA for CRISPRi
M1D3: Generate gRNA plasmid
M1D4: Journal club I
M1D5: Confirm gRNA sequence
M1D6: Induce CRISPRi system
M1D7: Complete CRISPRi experiment
M1D8: Journal club II

Assignments

Research article
Journal club presentation

References

paper for CRISPRi system

Notes for teaching faculty

Prep notes, M2(F16)
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