Difference between revisions of "20.109(F16):Module 2"
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'''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell], [http://be.mit.edu/directory/leslie-mcclain Leslie McClain] and [http://be.mit.edu/directory/maxine-jonas Maxine Jonas] | '''Instructors:''' [http://be.mit.edu/directory/noreen-lyell Noreen Lyell], [http://be.mit.edu/directory/leslie-mcclain Leslie McClain] and [http://be.mit.edu/directory/maxine-jonas Maxine Jonas] | ||
| − | '''TA:''' <br> | + | '''TA:''' Emily Clark <br> |
'''Lab manager:''' Hsinhwa Lee <br> | '''Lab manager:''' Hsinhwa Lee <br> | ||
==Overview== | ==Overview== | ||
| − | In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in ''E. coli''. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of ''E. coli'' such that either ethanol or | + | In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in ''E. coli''. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of ''E. coli'' such that either ethanol or lactate production is increased. |
| − | [[Image: |thumb|center|600px|Experimental overview for Module 2]] | + | [[Image:Fa16 M2 overview v2.png |thumb|center|600px|Experimental overview for Module 2]] |
<br style="clear:both" /> | <br style="clear:both" /> | ||
==Lab links: day by day== | ==Lab links: day by day== | ||
| − | [[20.109(F16):Complete in silico cloning (Day1)| | + | [[20.109(F16):Complete in silico cloning (Day1)| M2D1: Complete ''in silico'' cloning]]<br> |
| − | [[20.109(F16):Design gRNA for CRISPRi (Day2)| | + | [[20.109(F16):Design gRNA for CRISPRi (Day2)| M2D2: Design gRNA for CRISPRi]]<br> |
| − | [[20.109(F16):Generate gRNA plasmid (Day3)| | + | [[20.109(F16):Generate gRNA plasmid (Day3)| M2D3: Generate gRNA plasmid]]<br> |
| − | [[20.109(F16):Journal club I (Day4)| | + | [[20.109(F16):Journal club I (Day4)| M2D4: Journal club I]]<br> |
| − | [[20.109(F16):Confirm gRNA sequence (Day5)| | + | [[20.109(F16):Confirm gRNA sequence (Day5)| M2D5: Confirm gRNA sequence]]<br> |
| − | [[20.109(F16): | + | [[20.109(F16):Journal club II (Day 6) | M2D6: Journal club II]]<br> |
| − | [[20.109(F16): | + | [[20.109(F16):Induce CRISPRi system (Day7)| M2D7: Induce CRISPRi system]]<br> |
| − | [[20.109(F16): | + | [[20.109(F16)::Measure fermentation products (Day8)| M2D8: Measure fermentation products]] |
==Assignments== | ==Assignments== | ||
| − | [[20.109(F16): Research article | Research article]]<br> | + | [[20.109(F16): Research article | Research article]]<br> |
| − | [[20.109(F16): Journal club | + | [[20.109(F16):Journal club II (Day 6) | Journal club presentation]] |
==References== | ==References== | ||
| − | + | [[Media:CRISPRi NatMeth2013.pdf| CRISPR interference (CRISPRi) for sequence-specific control of gene expression]], (2013) by Larson ''et al.'' | |
==Notes for teaching faculty== | ==Notes for teaching faculty== | ||
| − | [[20.109(F16): | + | [[20.109(F16): TA notes for M2| F16 notes for M2]] |
Latest revision as of 19:39, 3 November 2016
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas
TA: Emily Clark
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or lactate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence
M2D6: Journal club II
M2D7: Induce CRISPRi system
M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression, (2013) by Larson et al.
