Difference between revisions of "20.109(F16):Module 2"
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[[20.109(F16):Journal club II (Day 6) | M2D6: Journal club II]]<br> | [[20.109(F16):Journal club II (Day 6) | M2D6: Journal club II]]<br> | ||
[[20.109(F16):Induce CRISPRi system (Day7)| M2D7: Induce CRISPRi system]]<br> | [[20.109(F16):Induce CRISPRi system (Day7)| M2D7: Induce CRISPRi system]]<br> | ||
| − | [ | + | [http://openwetware.org/wiki/20.109(F16):Measure_fermentation_products_(Day8) | M2D8: Measure fermentation products]<br> |
==Assignments== | ==Assignments== | ||
| − | [ | + | [http://openwetware.org/wiki/20.109(F16):_Research_article | Research article]<br> |
[[20.109(F16):Journal club II (Day 6) | Journal club presentation]] | [[20.109(F16):Journal club II (Day 6) | Journal club presentation]] | ||
==References== | ==References== | ||
| − | + | [[Media:CRISPRi NatMeth2013.pdf| CRISPR interference (CRISPRi) for sequence-specific control of gene expression]], (2013) by Larson et al. | |
==Notes for teaching faculty== | ==Notes for teaching faculty== | ||
| − | [ | + | [http://openwetware.org/wiki/20.109(F16):_Prep_notes_for_module_2 | Prep notes, M2(F16)] |
Revision as of 15:31, 28 July 2016
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas
TA:
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or lactate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence
M2D6: Journal club II
M2D7: Induce CRISPRi system
| M2D8: Measure fermentation products
Assignments
| Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression, (2013) by Larson et al.
