Difference between revisions of "20.109(F16):Module 2"
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[[20.109(F16): Research article | Research article]]<br> | [[20.109(F16): Research article | Research article]]<br> | ||
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==References== | ==References== | ||
Revision as of 15:16, 28 July 2016
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas
TA:
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or lactate production is increased.
Lab links: day by day
M1D1: Complete in silico cloning
M1D2: Design gRNA for CRISPRi
M1D3: Generate gRNA plasmid
M1D4: Journal club I
M1D5: Confirm gRNA sequence
M1D6: Journal club II
M1D7: Induce CRISPRi system
M1D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
paper for CRISPRi system
