Difference between revisions of "20.109(F16):Module 2"
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[[20.109(F16):Confirm gRNA sequence (Day5)| M1D5: Confirm gRNA sequence]]<br> | [[20.109(F16):Confirm gRNA sequence (Day5)| M1D5: Confirm gRNA sequence]]<br> | ||
[[20.109(F16):Induce CRISPRi system (Day6)| M1D6: Induce CRISPRi system]]<br> | [[20.109(F16):Induce CRISPRi system (Day6)| M1D6: Induce CRISPRi system]]<br> | ||
| − | [[20.109(F16): | + | [[20.109(F16):Measure fermentation products (Day7)| M1D7: Measure fermentation products]]<br> |
[[20.109(F16):Journal club II (Day 8) | M1D8: Journal club II]]<br> | [[20.109(F16):Journal club II (Day 8) | M1D8: Journal club II]]<br> | ||
Revision as of 15:02, 26 July 2016
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas
TA:
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or acetate production is increased.
[[Image: |thumb|center|600px|Experimental overview for Module 2]]
Lab links: day by day
M1D1: Complete in silico cloning
M1D2: Design gRNA for CRISPRi
M1D3: Generate gRNA plasmid
M1D4: Journal club I
M1D5: Confirm gRNA sequence
M1D6: Induce CRISPRi system
M1D7: Measure fermentation products
M1D8: Journal club II
Assignments
Research article
Journal club presentation
References
paper for CRISPRi system
