20.109(F19):Module 1
Contents
Module 1
Lecturer: Bevin Engelward
Instructors: Noreen Lyell, Leslie McClain and Becky Meyer
TAs: Colin Kim and Shelbi Parker
Lab manager: Hsinhwa Lee
Overview
Lab links: day by day
M1D1: Practice cell culture and begin gamma-H2AX assay
M1D2: Treat cells for gamma-H2AX assay and prepare CometChip for loading experiment
M1D3: Complete gamma-H2AX assay and perform loading experiment
M1D4: Load cells into CometChip and apply treatments for DNA damage experiment
M1D5: Complete DNA damage experiment
M1D6: Image CometChip
M1D7: Practice statistical analysis methods and complete data analysis
Assignments
Data summary
Mini-presentation
References
CometChip: A high-throughput 96-well platform for measuring DNA damage in microarrayed human cells. Journal of Visualized Experiments. (2014) 92: 1-11.
- A video of the procedure is linked here.
CometChip: Single-cell microarray for high-throughput detection of DNA damage. Methods in Cell Biology. (2012) 112: 247-268.