20.109(F16): Laboratory Fundamentals of Biological Engineering
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to experiments and techniques in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench -- ones that will increase the likelihood of success in your work and ensure the health and safety of you and those around you. By the end of the semester, you should also be aware of good scientific practice, having had some experience with scientific writing, notebook keeping, and orally presenting data and your ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
Schedule details:
Lecture: T/R 11-12 (16-220)
Laboratory sections: T/R 1-5 or W/F 1-5 (56-322)
MODULE
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DAY
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DATE
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LECTURER
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LABORATORY EXPERIMENTS
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ASSIGNMENTS
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|
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R/F Sept 8/9
|
NLL Orientation lecture
|
Orientation Prelab T/R Prelab W/F
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|
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1
|
1
|
T/W Sept 13/14
|
BE
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Prepare microwell array and practice tissue culture Prelab T/R Prelab W/F
|
Lab orientation quiz Homework due
|
1
|
2
|
R/F Sept 15/16
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BE
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Develop experiment to test loading variables and quantify growth rate Prelab T/R Prelab W/F
|
Homework due
|
1
|
3
|
T/W Sept 20/21
|
BE
|
Test role of biochemical factors in genomic stability Prelab T/R Prelab W/F
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Lab quiz Homework due
|
|
|
R/F Sept 22/23
|
BE
|
Lecture, but no laboratory Career fair student holiday
|
|
1
|
4
|
T/W Sept 27/28
|
NLL
|
Query inter-individual variability in exposure susceptibility Prelab T/R Prelab W/F
|
Lab quiz Homework due
|
1
|
5
|
R/F Sept 29/30
|
BE
|
Develop approach for sub-nuclear visualization of DNA damage Prelab T/R Prelab W/F
|
Homework due
|
1
|
6
|
T/W Oct 4/5
|
BE
|
Query DNA repair capacity in tumor cells
|
Lab quiz Homework due
|
1
|
7
|
R/F Oct 6/7
|
BE
|
Analysis of sub-nuclear foci
|
Homework due
|
|
|
T/W Oct 11/12
|
|
Columbus day holiday
|
Data Summary draft due Wed, Oct 12 at 5 pm
|
|
2
|
1
|
R/F Oct 13/14
|
NLL
|
Complete in silico cloning
|
Homework due Mini-presentation due Sat, Oct 15 at 10 pm
|
2
|
2
|
T/W Oct 18/19
|
NLL
|
Design gRNA for CRISPRi
|
Homework due
|
2
|
3
|
R/F Oct 20/21
|
NLL
|
Generate gRNA plasmid
|
Homework due Data Summary revision due Mon, Oct 24 at 5 pm
|
2
|
4
|
T/W Oct 25/26
|
NLL
|
Journal club I
|
Lab quiz Blog post due Tue, Oct 25 at 5pm
|
2
|
5
|
R/F Oct 27/28
|
NLL
|
Confirm gRNA sequence
|
Homework due
|
2
|
6
|
T/W Nov 1/2
|
NLL
|
Journal club II
|
Lab quiz
|
2
|
7
|
R/F Nov 3/4
|
NLL
|
Induce CRISPRi system
|
Homework due
|
2
|
8
|
T/W Nov 8/9
|
NLL
|
Measure fermentation products]
|
Lab quiz Homework due
|
|
|
R/F Nov 10/11
|
|
Veterans' day holiday
|
|
|
3
|
1
|
T/W Nov 15/16
|
AB
|
Growth of phage materials
|
Homework due
|
3
|
2
|
R/F Nov 17/18
|
AB
|
Purify active materials
|
Homework due Research article due Sat, Nov 19 at 5 pm Blog post due Sun, Nov 20 at 5pm
|
|
|
T/W Nov 22/23
|
AB
|
Lecture, but no laboratory
|
|
|
|
R/F Nov 24/25
|
|
Thanksgiving holiday
|
|
3
|
3
|
T/W Nov 29/30
|
AB
|
Cathode construction
|
Lab quiz Homework due
|
3
|
4
|
R/F Dec 1/2
|
AB
|
TEM
|
Homework due
|
3
|
5
|
T/W Dec 6/7
|
AB
|
Battery assembly and testing
|
Lab quiz Homework due Blog post due Wed, Dec 7 at 10pm
|
3
|
6
|
R/F Dec 8/9
|
|
Research proposal presentations
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Research proposal presentation slides due Thu/Fri, Dec 8/9 at 1 pm
|
|
|
|
T Dec 13
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Feedback and celebratory lunch
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Biomaterials engineering mini-report due Mon, Dec 12 at 10 pm Blog post due Wed, Dec 14 at 10pm
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