Difference between revisions of "20.109(F20): Fall 2020 schedule"
Becky Meyer (Talk | contribs) |
Becky Meyer (Talk | contribs) |
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| T/W Sep 8/9 | | T/W Sep 8/9 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M1D2 | Prepare and treat cells for repair foci experiment]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M1D2 | Prepare and treat cells for repair foci experiment]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M1D2|Homework due]] | | [[20.109(F20):Homework#Due_M1D2|Homework due]] | ||
|-- | |-- | ||
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| R/F Sep 10/11 | | R/F Sep 10/11 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M1D3 | Use immunoflourescence staining to assess repair foci experiment]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M1D3 | Use immunoflourescence staining to assess repair foci experiment]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M1D3|Homework due]] | | [[20.109(F20):Homework#Due_M1D3|Homework due]] | ||
|-- | |-- | ||
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| T/W Sep 15/16 | | T/W Sep 15/16 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M1D4 | Image repair foci experiment and quantify results]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M1D4 | Image repair foci experiment and quantify results]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M1D4|Homework due]] | | <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M1D4|Homework due]] | ||
|-- | |-- | ||
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| R/F Sep 17/18 | | R/F Sep 17/18 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M1D5 | Treat cells and perform high-throughput genome damage assay]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M1D5 | Treat cells and perform high-throughput genome damage assay]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M1D5|Homework due]] | | [[20.109(F20):Homework#Due_M1D5|Homework due]] | ||
|-- | |-- | ||
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| T/W Sep 22/23 | | T/W Sep 22/23 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M1D6 | Image and analyze high-throughput genome damage assay]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M1D6 | Image and analyze high-throughput genome damage assay]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M1D6|Homework due]] | | [[20.109(F20):Homework#Due_M1D6|Homework due]] | ||
|-- | |-- | ||
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| R/F Sep 24/25 | | R/F Sep 24/25 | ||
| [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/bevin-p-engelward BE]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M1D7 | Complete data analysis using statistical methods]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M1D7 | Complete data analysis using statistical methods]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M1D7|Homework due]] | | <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M1D7|Homework due]] | ||
|-- | |-- | ||
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| T/W Sep 29/30 | | T/W Sep 29/30 | ||
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M2D1 | Complete in silico cloning of protein expression plasmid]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M2D1 | Complete in silico cloning of protein expression plasmid]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M2D1|Homework due]] | | [[20.109(F20):Homework#Due_M2D1|Homework due]] | ||
|-- | |-- | ||
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| R/F Oct 1/2 | | R/F Oct 1/2 | ||
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M2D2 | Perform protein purification protocol]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M2D2 | Perform protein purification protocol]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M2D2| Homework due]]<br>[[20.109(F20):Data Summary| <font color = #b3251c>'''Data Summary draft due'''</font color>]] Sun, Oct 4 at 10 pm<br>[ Blog post due] Mon, Oct 5 at 10 pm | | [[20.109(F20):Homework#Due_M2D2| Homework due]]<br>[[20.109(F20):Data Summary| <font color = #b3251c>'''Data Summary draft due'''</font color>]] Sun, Oct 4 at 10 pm<br>[ Blog post due] Mon, Oct 5 at 10 pm | ||
|-- | |-- | ||
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| T/W Oct 6/7 | | T/W Oct 6/7 | ||
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M2D3 | Assess purity and concentration of purified protein]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M2D3 | Assess purity and concentration of purified protein]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M2D3|Homework due]] | | [[20.109(F20):Homework#Due_M2D3|Homework due]] | ||
|-- | |-- | ||
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| R/F Oct 8/9 | | R/F Oct 8/9 | ||
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M2D4 | Prepare small molecule microarray (SMM) slides with purified protein]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M2D4 | Prepare small molecule microarray (SMM) slides with purified protein]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M2D4|Homework due]]<br>[[20.109(F20):Mini-presentation| <font color = #b3251c>'''Mini-presentation due'''</font color>]] Sun, Oct 11 at 10 pm | | <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M2D4|Homework due]]<br>[[20.109(F20):Mini-presentation| <font color = #b3251c>'''Mini-presentation due'''</font color>]] Sun, Oct 11 at 10 pm | ||
|-- | |-- | ||
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| R/F Oct 15/16 | | R/F Oct 15/16 | ||
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M2D5 | Scan SMM slides to identify putative small molecule binders]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M2D5 | Scan SMM slides to identify putative small molecule binders]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M2D5|Homework due]]<br> | | [[20.109(F20):Homework#Due_M2D5|Homework due]]<br> | ||
|-- | |-- | ||
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| T/W Oct 27/28 | | T/W Oct 27/28 | ||
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M2D6 | Analyze SMM data to confirm putative small molecule binders]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M2D6 | Analyze SMM data to confirm putative small molecule binders]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M2D6|Homework due]]<br> | | [[20.109(F20):Homework#Due_M2D6|Homework due]]<br> | ||
|-- | |-- | ||
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| R/F Oct 29/30 | | R/F Oct 29/30 | ||
| [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/jacquin-c-niles JN]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M2D7 | Examine putative small molecule binders for common features]] ??<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M2D7 | Examine putative small molecule binders for common features]] ??<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M2D7|Homework due]]<br> | | <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M2D7|Homework due]]<br> | ||
|-- | |-- | ||
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| T/W Nov 3/4 | | T/W Nov 3/4 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M3D1 | Research project scope and experimental strategy]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M3D1 | Research project scope and experimental strategy]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M3D1|Homework due]]<br> | | [[20.109(F20):Homework#Due_M3D1|Homework due]]<br> | ||
|-- | |-- | ||
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| R/F Nov 5/6 | | R/F Nov 5/6 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M3D2 | Align gRNA sequences with genomic targets]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M3D2 | Align gRNA sequences with genomic targets]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M3D2|Homework due]] | | [[20.109(F20):Homework#Due_M3D2|Homework due]] | ||
|-- | |-- | ||
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| R/F Nov 12/13 | | R/F Nov 12/13 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M3D3 | Analyze ethanol yield data]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M3D3 | Analyze ethanol yield data]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M3D3|Homework due]]<br> | | <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M3D3|Homework due]]<br> | ||
|-- | |-- | ||
Line 163: | Line 163: | ||
| R/F Nov 19/20 | | R/F Nov 19/20 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M3D4 | Examine features in gRNA-targeted genomic sequences]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M3D4 | Examine features in gRNA-targeted genomic sequences]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| [[20.109(F20):Homework#Due_M3D4|Homework due]] | | [[20.109(F20):Homework#Due_M3D4|Homework due]] | ||
|-- | |-- | ||
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| T/W Dec 1/2 | | T/W Dec 1/2 | ||
| [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | | [http://be.mit.edu/directory/noreen-lyell NLL]<br> [https://mit.zoom.us/j/93419708072 Zoom access] <br> | ||
− | | [[20.109(F20):M3D5 | Design optimized gRNA sequence for enhanced ethanol production]]<br>[https://mit.zoom.us/j/ | + | | [[20.109(F20):M3D5 | Design optimized gRNA sequence for enhanced ethanol production]]<br>[https://mit.zoom.us/j/97716476442 T/R Zoom access], [ T/R prelab video], [[ T/R prelab slides]] <br> [https://mit.zoom.us/j/96606548971 W/F Zoom access], [ W/F prelab video], [[ W/F prelab slides]] <br> |
| <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M3D5|Homework due]] | | <font color= #829c58 >'''Laboratory quiz'''</font color><br>[[20.109(F20):Homework#Due_M3D5|Homework due]] | ||
|-- | |-- |
Revision as of 22:49, 4 September 2020
Welcome to 20.109! It is our goal to make this class a useful and fun introduction to the experiments and techniques used in biological engineering. Though there is not time enough to show you everything needed to do research, after this class you will feel confident and familiar with some fundamental experimental approaches and laboratory protocols. You will develop good habits at the bench, which will increase the likelihood of success in your work and ensure the health and safety of you and your labmates. By the end of the semester, you will also be well-versed in good scientific practices - through your experience with scientific writing, notebook keeping, and orally presenting data and novel ideas. All of us involved in teaching 20.109 hope you will find it a satisfying challenge and an exciting experience that has lasting value.
SCHEDULE DETAILS:
Lecture times: Tuesday (T) and Thursday (R) 11 - 12 pm
Laboratory section times: Tuesday (T) and Thursday (R) 3 - 5 pm or Wednesday (W) and Friday (F) 3 - 5 pm