Difference between revisions of "20.109(F16):Module 2"
Noreen Lyell (Talk | contribs) (→Assignments) |
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==Lab links: day by day== | ==Lab links: day by day== | ||
− | [[20.109(F16):Complete in silico cloning (Day1)| M2D1: Complete in silico cloning]]<br> | + | [[20.109(F16):Complete in silico cloning (Day1)| M2D1: Complete ''in silico'' cloning]]<br> |
[[20.109(F16):Design gRNA for CRISPRi (Day2)| M2D2: Design gRNA for CRISPRi]]<br> | [[20.109(F16):Design gRNA for CRISPRi (Day2)| M2D2: Design gRNA for CRISPRi]]<br> | ||
[[20.109(F16):Generate gRNA plasmid (Day3)| M2D3: Generate gRNA plasmid]]<br> | [[20.109(F16):Generate gRNA plasmid (Day3)| M2D3: Generate gRNA plasmid]]<br> |
Revision as of 11:40, 29 September 2016
Contents
Module 2
Lecturer: Noreen Lyell
Instructors: Noreen Lyell, Leslie McClain and Maxine Jonas
TA: Emily Clark
Lab manager: Hsinhwa Lee
Overview
In this module you will use a modified Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system to modulate transcription in E. coli. Specifically, you will use a CRISPR-based interference system to target a gene within the fermentation pathway of E. coli such that either ethanol or lactate production is increased.
Lab links: day by day
M2D1: Complete in silico cloning
M2D2: Design gRNA for CRISPRi
M2D3: Generate gRNA plasmid
M2D4: Journal club I
M2D5: Confirm gRNA sequence
M2D6: Journal club II
M2D7: Induce CRISPRi system
| M2D8: Measure fermentation products
Assignments
Research article
Journal club presentation
References
CRISPR interference (CRISPRi) for sequence-specific control of gene expression, (2013) by Larson et al.