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• 20.109(F16):Purify active materials (Day2)
  Today Dr. Sean Clarke from the BE Communication Lab will join us to provide some tips for ...y mixing 500 μL of phage (at 1.7 x 10¹³ pfu/mL) with 500 μL of a 10 mM (NH₄)₂Fe(SO₄)₂ solut
  6 KB (1,002 words) - 18:40, 15 November 2016
• 20.109(F16):Test comet chip loading variables (Day2)
  Our communication instructors, Dr. Sean Clarke and Dr. Diana Chien, will join us today for a workshop on designing ... If the calculated volumes are less than 50 μL or greater than 400 μL, consult the teaching faculty with how to move forward.
  15 KB (2,594 words) - 17:33, 22 September 2016
• 20.109(F16):Complete immuno-fluorescence assay (Day6)
  Our communication instructors, Dr. Sean Clarke and Dr. Diana Chien, will join us today for a workshop on writing an #Permeabilize the cells by adding 500 μL of the Triton X-100 solution to each well and incubate for 10 min at room t
  9 KB (1,404 words) - 12:00, 5 October 2016
• 20.109(F16):Generate gRNA plasmid (Day3)
  Our communication instructors, Dr. Sean Clarke and Dr. Diana Chien, will join us today for a workshop on preparing #Calculate the volume of your stock that is required to prepare a 100 μL of solution that contains your gRNA oligo at a concentration of 10 μM.
  11 KB (1,747 words) - 20:42, 14 November 2016
• 20.109(F16):Induce CRISPRi system (Day7)
  ...e encoding Cas9 within pdCas9 is regulated by an inducible promoter (p_LtetO-1). An inducible promoter is 'off' unless the appropriate molecu Our communication instructor Dr. Sean Clarke will join us today for a workshop on organizing and writing your M2
  14 KB (2,216 words) - 19:38, 3 November 2016
• 20.109(S17):Purify active materials (Day2)
  Today Dr. Sean Clarke from the BE Communication Lab will join us to provide some tips for ...d by mixing 500 μL of phage (at 7 x 10¹³ pfu/mL) with 50 μL of a 100 mM (NH₄)₂Fe(SO₄)₂ solu
  6 KB (1,004 words) - 14:34, 24 April 2017
• 20.109(F17):Generate gRNA plasmid (Day3)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on prepar #Calculate the volume of your stock that is required to prepare a 100 μL of solution that contains your gRNA oligo at a concentration of 10 μM.
  9 KB (1,451 words) - 16:32, 19 October 2017
• 20.109(F17):Induce CRISPRi system (Day7)
  ...e encoding Cas9 within pdCas9 is regulated by an inducible promoter (p_LtetO-1). An inducible promoter is 'off' unless the appropriate molecu Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on organi
  14 KB (2,222 words) - 20:39, 30 October 2017
• 20.109(F17):Purify active materials (Day2)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today to provide some tips for ...y mixing 500 μL of phage (at 1.7 x 10¹³ pfu/mL) with 267 μL of a 25 mM (NH₄)₂Fe(SO₄)₂ solut
  6 KB (1,012 words) - 19:28, 16 November 2017
• 20.109(F17):Complete biochemical experiment (Day5)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on writin #In the 'no enzyme treatment' control CometChip, add 50 μL of 1X complete Fpg reaction buffer to each well that was loaded and treated
  12 KB (1,953 words) - 12:41, 29 September 2017
• 20.109(S18):Evaluate protein purity and concentration (Day4)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on writin #Retrieve the 15 μL aliquots of your IPTG-induced and the uninduced cell lysates you prepared d
  12 KB (1,851 words) - 20:02, 26 February 2018
• 20.109(S18):Purify phage-based active (cathode) material (Day2)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today to provide some tips for ...y mixing 500 μL of phage (at 2 x 10¹³ phage/mL) with 267 μL of a 25 mM (NH₄)₂Fe(SO₄)₂ solut
  6 KB (1,017 words) - 16:41, 24 April 2018
• 20.109(S18):Confirm ligand binding using differential scanning fluorimetry assay (Day6)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on writin | 1.2 μg/10 μL
  11 KB (1,775 words) - 13:16, 8 March 2018
• 20.109(S18):Investigate RNA-seq data using public databases (Day5)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on prepar #Add 100 μL of Buffer PB to each cDNA preparation.
  10 KB (1,557 words) - 16:09, 23 March 2018
• 20.109(F18):Generate gRNA plasmid (Day3)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on prepar #Calculate the volume of your stock that is required to prepare a 100 μL of solution that contains your gRNA oligo at a concentration of 10 μM.
  9 KB (1,441 words) - 20:11, 15 October 2018
• 20.109(F18):Induce CRISPRi system (Day7)
  ...e encoding Cas9 within pdCas9 is regulated by an inducible promoter (p_LtetO-1). An inducible promoter is 'off' unless the appropriate molecu Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on organi
  14 KB (2,223 words) - 16:46, 1 November 2018
• 20.109(F18):Purify phage-based active (cathode) material (Day2)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today to provide some tips for ...y mixing 500 μL of phage (at 2 x 10¹³ phage/mL) with 267 μL of a 25 mM (NH₄)₂Fe(SO₄)₂ solut
  6 KB (989 words) - 18:13, 13 November 2018
• 20.109(F18):Prepare and treat cells for genomic instability experiment (Day3)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on design #Using the P1000, pipet up 1000 μL of molten agarose from the tube.
  9 KB (1,587 words) - 22:12, 20 September 2018
• 20.109(S19):Purify phage-based active (cathode) material (Day2)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today to provide some tips for #Vortex the nanowire pool for at least 1 minute and immediately remove 10 &mu;L of the nanowire suspension to place on the <b> silver/black side (not the c
  5 KB (851 words) - 17:00, 24 April 2019
• 20.109(S19):Confirm ligand binding using differential scanning fluorimetry assay (Day6)
  Our communication instructors, Dr. Sean Clarke and Dr. Prerna Bhargava, will join us today for a workshop on writin |1 &mu;g/30 &mu;L reaction
  10 KB (1,695 words) - 20:09, 5 March 2019